HPLC同时测定复方鱼腥草片中4个有效成分的含量

Simultaneous Determination of 4 Effective Components in Fufang Yuxingcao Tablets by HPLC

关键词:  复方鱼腥草片  绿原酸  连翘酯苷A  槲皮苷  黄芩苷  质量控制  高效液相色谱法

Key words:  Fufang Yuxingcao tablets  chlorogenic acid  forsythoside A  quercitroside  baicalin  quality control  HPLC

摘要:

目的 建立HPLC同时测定复方鱼腥草片中绿原酸、连翘酯苷A、槲皮苷和黄芩苷含量的方法。方法 采用CAPCELL PAK MG（4.6 mm×250 mm，5μm）色谱柱，流动相为乙腈（A）-0.1%磷酸溶液（B），梯度洗脱（0~12 min，9% A；12~15 min，9%→15% A；15~32 min，15% A；32~48 min，15%→29% A；48~60 min，29% A），流速1.0 mL·min^-1，柱温35℃，检测波长：330 nm。结果 绿原酸、槲皮苷浓度在2~100 μg·mL^-1内，连翘酯苷A浓度在4~200 μg·mL^-1内，黄芩苷浓度在12~600 μg·mL^-1内，与峰面积均呈良好的线性关系（r=0.999 9）；平均回收率（n=6）分别为98.0%，99.8%，99.1%和98.3%，RSD均<2.0%。结论 建立的HPLC可实现同时测定绿原酸、连翘酯苷A、槲皮苷和黄芩苷4种有效成分，方法快速、简便、结果准确，可为复方鱼腥草片提供更全面、可靠的质量控制方法。

Abstract:

OBJECTIVE To establish an HPLC method for simultaneous determination of chlorogenic acid, forsythoside A, quercitroside and baicalin in Fufang Yuxingcao tablets. METHODS The samples were separated on a CAPCELL PAK MG (4.6 mm×250 mm, 5 μm) column, by a gradient elution using acetonitrile(A) and 0.1% phosphoric acid(B) (0-12 min, 9%A; 12-15 min, 9%→15%A; 15-32 min, 15%A; 32-48 min, 15%→29%A; 48-60 min, 29%A) as mobile phase at a flow rate of 1.0 mL·min^-1. The column temperature was set at 35℃. The wavelength was set on 330 nm. RESULTS The linear ranges of chlorogenic acid, quercitroside, forsythoside A and baicalin were 2-100 μg·mL^-1, 2-100 μg·mL^-1, 4-200 μg·mL^-1 and 12-600 μg·mL^-1(r=0.999 9), the average recoveries(n=6) were 98.0%, 99.8%, 99.1% and 98.3%, respectively, and RSDs were < 2.0%. CONCLUSION A rapid, simple, accurate HPLC method is successfully established for simultaneous determine 4 effective components in Fufang Yuxingcao tablets. The method herein described is helpful for the quality control of Fufang Yuxingcao tablets.