清脑片对沙鼠脑缺血再灌注的保护作用及其机制研究

Protective Effects and Mechanism of Qingnao Tablet on Cerebral Ischemia Reperfusion in Gerbils

关键词:  清脑片  脑缺血再灌注  沙鼠  酶活力  保护作用

Key words:  Qingnao tablet  cerebral ischemia reperfusion  gerbil  enzyme activity  protective effect

摘要:

目的 探究清脑片对沙鼠脑缺血再灌注的保护作用及其机制。方法 采用双侧颈总动脉结扎10 min再灌注60 min，建立沙鼠脑缺血模型，观察高、中、低剂量清脑片组（1.5，0.75，0.375 g·kg^-1）对沙鼠脑组织中白介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）、兴奋性氨基酸谷氨酸（Glu）、天门冬氨酸（Asp）、血栓烷素B₂（TXB₂）、丙二醛（MDA）含量及ATP活性和NF-кB免疫阳性表达的影响。结果 与模型组比较，高、中剂量清脑片可显著降低脑组织中IL-1β、TNF-α、Glu、Asp、TXB₂、MDA的含量及NF-κB免疫阳性表达水平（P<0.01或P<0.05），显著提高脑组织中Na⁺-K⁺-ATP、Mg²⁺-ATP、Ca²⁺-ATP、Ca²⁺-Mg²⁺-ATP的酶活性（P<0.01或P<0.05）。结论 清脑片对沙鼠脑缺血再灌注有明显的保护作用，其作用机制可能与抑制体内炎症因子、过氧化能力及提高酶活力有关。

Abstract:

OBJECTIVE To explore the protective effect and mechanism of Qingnao tablet on cerebral ischemia reperfusion in gerbils. METHODS Gerbil's bilateral carotid arteries were occluded for 10 min and then reperfused 60 min to make cerebral ischemia gerbil model. The effects of large, medium and small dose of Qingnao tablet group(1.5, 0.75, 0.375 g·kg^-1) on interleukin-1β(IL-1β), tumor necrosis factor alpha (TNF-α), excitatory amino acid glutamate (Glu), aspartic acid (Asp), thromboxane B₂(TXB₂), malondialdehyde (MDA) content and the activity of ATP and immunoreactive expression of NF-κB in gerbils were observed. RESULTS Compared with model group, IL-1, TNF-α, Glu, Asp, TXB₂ brain tissue, the content of MDA and NF-κB positive expression were significantly decreased by large and medium dose of Qingnao tablet (P<0.01 or P<0.05); Then the activity of brain tissue Na⁺-K⁺-ATP, Mg²⁺-ATP Ca²⁺-ATP, Ca²⁺-Mg²⁺-ATP were significantly increased (P<0.01 or P<0.05). CONCLUSION Qingnao tablet on gerbil cerebral ischemia reperfusion injury has obvious protective effect, its mechanism may be related to the inhibition of peroxidation of inflammatory cytokines, and improve enzyme activity.