| 引用本文: | 叶爱琴,吴凡,蔡田恬,蒋芳萍,白海波,傅旭春.豨莶草的提取工艺研究[J].中国现代应用药学,2017,34(7):988-991. |
| YE Aiqin,WU Fan,CAI Tiantian,JIANG Fangping,BAI Haibo,FU Xuchun.Study on the Extraction Process of Effective Fractions from Siegesbeckiae Herba[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(7):988-991. |
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| 豨莶草的提取工艺研究 |
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叶爱琴1, 吴凡2, 蔡田恬2, 蒋芳萍3, 白海波3, 傅旭春4
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1.温州市中医院, 浙江 温州 325000;2.浙江省中医院药剂科, 杭州 310006;3.杭州华东医药集团新药研究院有限公司, 杭州 310012;4.浙江大学城市学院药物研究所, 杭州 310015
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| 摘要: |
| 目的 筛选豨莶草的最佳提取工艺。方法 用HPLC测定提取物中奇壬醇含量；以豨莶草所含奇壬醇为筛选指标，用正交设计法优化乙醇提取工艺。结果 乙醇溶液提取的奇壬醇得率明显高于水提取；豨莶草药材的最佳乙醇提取工艺为： 8倍量70%乙醇，浸润2 h，加热回流提取3次（第1次2.5 h，第2次1 h，第3次1 h）。结论 乙醇溶液作为豨莶草的提取溶媒优于水；优化后的醇提工艺合理可行。 |
| 关键词: 豨莶草 奇壬醇 提取工艺 正交设计 |
| DOI：10.13748/j.cnki.issn1007-7693.2017.07.012 |
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| Study on the Extraction Process of Effective Fractions from Siegesbeckiae Herba |
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YE Aiqin1, WU Fan2, CAI Tiantian2, JIANG Fangping3, BAI Haibo3, FU Xuchun4
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1.Wenzhou Traditional Chinese Medical Hospital, Wenzhou 325000, China;2.Zhejiang Traditional Chinese Medical Hospital, Hangzhou 310006, China;3.Hangzhou Huadong Medicine Group Pharmaceutical Research Institute Co., Ltd., Hangzhou 310012, China;4.Institute of Materia Medica, Zhejiang University City College, Hangzhou 310015, China
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| Abstract: |
| OBJECTIVE To screen the optimum extraction process of effective fractions from Siegesbeckiae Herba.METHODS HPLC was used to determine the content of kirenol in Siegesbeckiae Herba extract.The ethanol extraction process was optimized by orthogonal test when kirenol content was selected as index.RESULTS The kirenol yield obtained from Siegesbeckiae Herba was higher using ethanol solution as solvent than water.The optimum extraction process of Siegesbeckiae Herba was to extract three times (the first 2.5 h,the second 1 h,the third 1 h) using eightfold 70% ethanol after infiltrating for 2 h.CONCLUSION Ethanol solution is better than water as solvent for extraction of kirenol.The optimised extraction process was reasonable and feasible. |
| Key words: Siegesbeckiae Herba kirenol extraction process orthogonal test |
