双环己铜草酰二腙诱导的脱髓鞘小鼠造模方法的改进

Improvement on Mice Model of Cuprizone-induced Demyelination

关键词:  双环己铜草酰二腙  脱髓鞘  灌胃

Key words:  cuprizone  demyelination  intragastric administration

摘要:

目的 改进双环己铜草酰二腙（cuprizone，CPZ）诱导脱髓鞘小鼠模型的制备方法，为治疗脱髓鞘疾病提供更精准快速的动物模型。方法 取60只18~20 g的C57BL/6小鼠，分成空白对照组，CPZ灌胃组（共4组，300 mg·kg^-1，qd，bid，tid，qid），CPZ饲喂组（即传统模型组），每组10只。空白对照组及CPZ灌胃组给予正常饲料，CPZ饲喂组给予0.2% CPZ混合鼠饲料连续饲喂6周构建小鼠脱髓鞘模型，通过免疫组化和髓鞘染色技术LFB检测髓鞘脱失情况，并比较CPZ灌胃模型和传统模型诱导小鼠急性髓鞘脱失的差异。结果 作用3周后，4组CPZ灌胃组的小鼠脑胼胝体区髓鞘碱性蛋白MBP染色与对照组相比均明显减少，而传统模型中小鼠脑胼胝体区MBP在第3周并未显著改变，在第6周明显减少。LFB染色结果表明CPZ灌胃3周后小鼠脑胼胝体区染色显著减少。结论 改进后的模型（小鼠CPZ灌胃模型）小鼠在第3周即能出现脱髓鞘现象，能有效缩短时间，具有显著优越性，且CPZ剂量可控，保证模型的精准性。

Abstract:

OBJECTIVE To improve the method of cuprizone(CPZ)-induced demyelinating mice model, and provide a more accurate and rapid animal model for the study on treatment of demyelinating disease. METHODS Sixty mice were averagely and randomly divided into control(i.g/d), CPZ induced model (300 mg·kg^-1 i.g/d, qd, bid, tid, qid) and CPZ traditional model (mixed with 0.2% cuprizone in the feed, and feeding 6 weeks), myelination was detected by immunohistochemical and LFB staining. The differences of acute demyelination in mice induced by CPZ gavage model and traditional model were compared. RESULTS The expression of myelin basic protein (MBP) was diminished significantly in CPZ-induced mice(i.g) for 3 weeks, but diminished in traditional demyelinating model for 6 weeks. The same results showed in LFB staining. CONCLUSION The improved mice model can shorten the time effectively and accurately, which showed obvious advantages.