苦豆子多糖对小鼠体内外免疫功能的调节作用

Effect of Polysaccharide from Sophora Alopecuroides on Regulating the Immune Function in Mice

关键词:  苦豆子多糖  脾淋巴细胞增殖  细胞免疫  体液免疫

Key words:  Sophora alopecuroides polysaccharide  spleen lymphocyte proliferation  cellular immune  humoral immune

摘要:

目的 考察苦豆子多糖对小鼠免疫功能的体内外调节作用。方法 MTT法体外观察苦豆子多糖在不同浓度对小鼠脾淋巴细胞增殖的影响；腹腔注射环磷酰胺建立免疫低下小鼠模型，通过碳粒廓清和血清溶血素实验测定非特异性免疫功能和体液免疫功能，测定小鼠巨噬细胞的碳粒廓清指数K、吞噬指数α和小鼠血清中溶血素的含量。结果 不同浓度苦豆子多糖可不同程度地促进小鼠脾淋巴细胞的增殖；苦豆子多糖能在一定程度上提高小鼠腹腔巨噬细胞的廓清能力、吞噬速度及血清中溶血素的含量，提高小鼠机体免疫能力。结论 苦豆子多糖能够通过多种免疫途径增强小鼠免疫功能。

Abstract:

OBJECTIVE To investigate the immune regulating effect of the polysaccharide from Sophora alopecuroides (SAP) in mice. METHODS The effects of SAP on the proliferation of splenic lymphocytes in mice were inspected by MTT. Nonspecific and humoral immune function of the immuno-suppression mice model which was induced by cyclophosphamide were evaluated by the carbon granular clearance test and hemolysin test. Furthermore, the carbon clearance index K, phagocytic index α and the content of hemolysin in mice serum were determined. RESULTS Different concentrations of SAP could promote the proliferation of splenic lymphocytes with various degrees in vitro. SAP could improve the immune function of mice through increasing the clearance ability, phagocytosis rate and the content of hemolysin. CONCLUSION SAP can enhance the immune function of mice through a variety of immune routes.