解酒饮保护小鼠急性酒精性肝损伤的研究

Study on Acute Alcohol Liver Injury Induced by Jiejiu Oral Liquid in Mice

关键词:  肝疾病  解酒饮  酒精  急性肝损伤

Key words:  liver diseases  Jiejiu oral liquid  alcohol  acute liver injury

摘要:

目的 探讨解酒饮对小鼠急性酒精性肝损伤的保护作用。方法 将小鼠随机分为正常对照组、联苯双酯组（11.7 mg·kg^-1）、模型对照组和解酒饮低、中、高剂量（6.25，12.5，25 g·kg^-1）组6组，每组10只，每天1次，连续14 d灌服给药。末次给药后1 h，除正常对照组外按12 ml·kg^-1剂量一次性灌胃给予56°北京红星二锅头造模。12 h后处死小鼠取血标本和肝组织标本，比较各组的肝脏指数，并对肝组织进行组织形态学检查，测定血清谷丙转氨酶（ALT）、谷草转氨酶（AST）的活性水平。结果 解酒饮可显著降低酒精诱导的急性肝损伤小鼠的血清ALT、AST水平，可减少肝中脂肪空泡，减轻炎症表现，急性肝损伤肝脏指数明显改善。结论 解酒饮对小鼠急性酒精性肝损伤有一定的保护作用。

Abstract:

OBJECTIVE to investigate the protective effect of Jiejiu Oral Liquid on acute alcoholic liver injury in mice. METHODS The mice were randomly divided into 6 groups, 10 rats in each group:normal group, bifendate group (11.7 mg·kg^-1), model group and low, medium, high dose(6.25, 12.5, 25 g·kg^-1) group. Mice were administered 1 times a day for 14 d. except for the normal control mice were given 56 degrees of Beijing Red Star Erguotou according to 12 ml·kg^-1 single dose by intragastric administration. twelve hours later, the mice were killed to take blood samples and liver tissue specimens. then compared the liver index, histological examination of liver tissue, serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity level. RESULTS the serum ALT, AST, the fatty vacuoles in liver and the inflammatory symptoms were significantly reduced in alcohol induced acute liver injury mice, liver index was significantly improved in acute liver injury. CONCLUSION Jiejiu Oral Liquid has a protective effect on acute alcoholic liver injury in mice.