姜黄素对四氯化碳诱导大鼠急性肝损害的保护作用

Protective Effects of Curcumine on CCl4-induced Acute Liver Injury in Rats

关键词:  姜黄素  四氯化碳  大鼠  急性肝损伤

Key words:  curcumin  CCl₄  rats  acute liver injury

摘要:

目的 研究姜黄素对四氯化碳诱导的大鼠急性肝损伤的保护作用及其机制。方法 60只健康♂SD大鼠随机分成6组，即正常对照组，肝损伤模型组，阳性药物对照水飞蓟素组（100 mg·kg^-1），姜黄素低剂量组（25 mg·kg^-1），姜黄素中剂量组（50 mg·kg^-1）和姜黄素高剂量组（100 mg·kg^-1）。隔天灌胃给药，共30 d；末次给药1 h后，腹腔注射20 mg·kg^-1 CCl₄玉米油溶液（2 mL·kg^-1）造模，禁食不禁水，12 h后乌拉坦麻醉。取下腔静脉血和肝脏后，分别检测大鼠血清中丙氨酸氨基转移酶（AST）及天门冬氨酸氨基转移酶（ASL）的活性，大鼠肝脏组织中血红素加氧酶Ⅰ（HO-1）及静脉血中HbCO的水平，在体外测定姜黄素清除DPPH自由基及ABTS自由基的能力。结果 与正常对照组相比，模型组血清中ALT、AST活性显著升高，肝组织中HO-1活性及静脉血中HbCO的含量显著降低，组织病理检查显示肝组织损伤明显增加。与模型组相比，姜黄素各剂量组可不同程度的降低血清AST及ASL的活性，增加肝脏组织中HO-1的活性及HbCO的水平，组织病理检查显示肝损伤有不同程度减轻。并且姜黄素具有清除DPPH自由基及ABTS自由基的能力。结论 姜黄素对CCl₄诱导的大鼠急性肝损伤具有一定保护作用，其机制可能与其自身抗氧化能力及诱导HO-1及HbCO有关。

Abstract:

OBJECTIVE To investigate the protective effect of curcumin on carbon tetrachloride-induced liver injury in rats and its underlying mechanism. METHODS Sixty ♂ SD rats were randomly divided into 6 groups as normal control group, model group, positive control silymarin group(100 mg·kg^-1), curcumin low dose group (25 mg·kg^-1), curcumin middle dose group (50 mg·kg^-1), curcumin high dose group (100 mg·kg^-1). They were given relevant medicines (i.g.) every other day for 30 d. One hours after the final treatment, CCl₄ dissolved in corn oil (20 mg·kg^-1) was administrated intraperitoneally (i.p.) to each group except control group. After the completion of the experiment, food and water were removed 12 h prior to sacrifice. The rats were anesthetized by urethane, and blood was collected from the hepatic portal vein. The activities of AST, ALT and HO-1, the HbCO content in the blood and the scavenging effects of curcumin on DPPH and ABTS were determined. RESULTS Compared with normal control group, the activities of ALT and AST in serum of model group were significantly increased, and the activity of HO-1 in liver tissue and the content of HbCO in venous blood were significantly decreased. Histopathological examination showed that liver injury was increased significantly. Compared with model group, pretreatment of rats with different concentration of curcumin decreased the serum levels of ALT and AST, increased the HO-1 activity and the content of HbCO in the liver, and the liver injury was alleviated in different degrees by histopathological examination. And curcumin exhibited strong scavenging effects on DPPH and ABTS free radicals. CONCLUSION The study demonstrates that curcumin has a potent hepatoprotective function against CCl₄-induced hepatic injuries in rats through the antioxidant effects and the inducing HO-1 and HbCO.