海桐种子醇提物中挥发性成分的GC-MS分析及抗内毒素活性测定

GC-MS Analysis of Volatile Components in Ethanol Extract from Seeds of Pittosporum Tobira Ait. and Determination of Anti-endotoxin Activity

关键词:  海桐种子  挥发性成分  GC-MS分析  抗内毒素作用

Key words:  seeds of Pittosporum tobira Ait.  volatile components in ethanol extract  GC-MS analysis  anti-endotoxin activity in vitro

摘要:

目的 研究海桐种子乙醇总提取物（ethanol extract from seeds of Pittosporum tobira Ait，EESPTA）的挥发性成分及相对含量，考察其抗内毒素活性。方法 以水蒸气蒸馏法提取EESPTA的挥发性成分，用气-质联用技术初步分析鉴定，并用峰面积归一化法测定各成分相对含量，采用终点基质显色法鲎实验检测其对细菌内毒素的清除效果，考察其抗内毒素活性。结果 共检测出并初步分析鉴定32个色谱峰，占该挥发性成分总量的99.82%。在EESPTA的挥发性成分中，1R，4S，7S，11R-2，2，4，8-四甲基三环[5.3.1.0（4，11）]十一碳-8-烯含量高达54.78%，其他含量较高的成分有2-亚甲基-胆甾烷-3-醇（7.27%）、蛇麻烷-1，6-二烯-3-醇（6.23%）、4-甲基-5-癸醇（4.11%）、绿花白千层醇（3.86%）、别香橙烯（3.80%）、反式-2，3-二甲基丙烯酸（3.50%）、二氢-噻喃-3，5-二醇-四氢-4-甲基-4-氮-二乙酸乙酯（2.04%）等成分。结论 EESPTA具有较明显的抗细菌内毒素活性，并呈浓度依赖关系。为进一步开发利用海桐药用价值提供了科学依据。

Abstract:

OBJECTIVE To analyze the volatile components in ethanol extract from seeds of Pittosporum tobira Ait.(EESPTA) and investigate the anti-endotoxin activity of EESPTA in vitro. METHODS The volatile components in EESPTA were extracted with steam distillation method and then analysized by GC-MS. The relative content of each component was calculated by peak area normalization. The anti-endotoxin activity of EESPTA in vitro was investigated by CEPTAL (chromogenic end-point tachypleus amebocyte lysate assay). RESULTS The 32 peaks were detected and then identified preliminary, which made up 99.82% of the total volatile components. The anti-endotoxin activity of EESPTA in vitro was observed in concentration-dependent manner from CEPTAL assay. The highest content constituent was 1R,4S,7S,11R-2,2,4,8-tetramethyltricyclo[5.3.1.0(4,11)] undec-8-ene (54.78%), and other relative high content constituents were 2-methylene-cholestan-3-ol(7.27%), humulane-1, 6-dien-3-ol(6.23%), 4-methyl-5-decanol(4.11%), viridiflorol(3.86%), Alloaromadendrene (3.80%), 2-methyl-2-butenoic acid(3.50%), et al. CONCLUSION EESPTA has obvious anti-endotoxin activity with a concentration dependence. This study provides a scientific basis for further development and utilization of Pittosporum tobira Ait..