丹酚酸B镁对兔急性心梗再灌注后无复流心肌损伤的保护作用

Protective Effect of Salvianolic Acid B Magnesium on the Myocardial Injury After Acute Myocardial Infarction and Reperfusion No-reflow in Rabbits

关键词:  急性心肌梗死  缺血再灌注损伤  无复流  丹酚酸B镁

Key words:  acute myocardial infarction  ischemial-reperfusion injury  no-reflow  salvianolic acid B magnesium

摘要:

目的 评价丹酚酸B镁（salvianolic acid B，Sal-B）对兔急性心肌梗死再灌注后心肌损伤的保护作用。方法 新西兰大白兔40只随机分成4组，即假手术组、心肌缺血再灌注（myocardial ischemia/reperfusion，MI/R）、再灌注低剂量组（Sal-B20 mg·kg^-1组）、再灌注高剂量组（Sal-B 60 mg·kg^-1组），每组10只。假手术组只开胸不结扎，其余3组结扎左室缘支90 min，切断结扎线120 min，建立MI/R模型。各组分别于结扎左心室缘支前5 min、结扎后90 min、再灌注120 min时取血，检测肌酸激酶同工酶（CK-MB）、心肌肌钙蛋白I （cTnI），并评估缺血范围、无复流范围及梗死区心肌范围。结果 结扎90 min后，MI/R组、Sal-B 20 mg·kg^-1组和Sal-B 60 mg·kg^-1组3组之间CK-MB、cTnI水平差异无统计学意义。再灌注120 min后，Sal-B 60 mg·kg^-1组的血清CK-MB、cTnI水平显著低于MI/R组、Sal-B 20 mg·kg^-1组，差异有统计学意义（P<0.05）。各组染色所测的冠脉结扎区心肌缺血范围基本一致。与MI/R组、Sal-B 20 mg·kg^-1组比，Sal-B 60 mg·kg^-1组可以显著减少无复流面积（P<0.05）和梗死面积（P<0.05），MI/R组和Sal-B 20 mg·kg^-1组之间差异无统计学意义。结论 Sal-B 60 mg·kg^-1能在一定程度上减轻心肌细胞结构损伤，缩小心肌梗死面积，减轻无复流的发生。

Abstract:

OBJECTIVE To evaluate the protective effects of salvianolic acid B magnesium (Sal-B) on the myocardial injury after acute myocardial infarction and reperfusion in rabbits. METHODS Forty New Zealand rabbits were divided randomly into four groups(n=10):sham operation group, myocardial ischemia/reperfusion(MI/R) group, Sal-B 20 mg·kg^-1 group (reperfusion with the treatment of low dose group), Sal-B 60 mg·kg^-1 group (reperfusion with the treatment of high dose group). The rabbits in the sham operation group were only opened chest without ligation, the others were subjected to 90 min of coronary occlusion followed by reperfusion for 120 min. The serum levels of CK-MB, cTnI were respectively detected in the former 5 min ligation and 90 min ligation, 120 min reperfusion. And to evaluate the area range of no-reflow, infracted area and risk area of the heart. RESULTS After 90 min ligation, serum contents of CK-MB, cTnI were no significant among MI/R group, Sal-B 20 mg·kg^-1, Sal-B 60 mg·kg^-1 group. Serum of CK-MB, cTnI contents in Sal-B 60 mg·kg^-1 group was lower than that in MI/R group and Sal-B 20 mg·kg^-1 group after 120 min of reperfusion (P<0.05). The ligation area(LA) measured by pathological evaluation were no significant difference in all groups. Compared with the MI/R group and Sal-B 20 mg·kg^-1 group, the degree of reduction in infarct size and morphological injury of myocardium were more significantly in the Sal-B 60 mg·kg^-1 group (P<0.05). The area of myocardial infarction in the MI/R group and Sal-B 20 mg·kg^-1 group were similar. CONCLUSION Sal-B 60 mg·kg^-1 can reduce myocardial cell structure damage, decrease no-reflow area and myocardial infarction size in experimental acute myocardial ischemial-reperfusion.