苯甲酰乌头原碱配伍芍药苷的抗炎镇痛作用研究

Anti-inflammation and Analgesia Effects of Combination Therapy with Benzoylaconine and Paeoniflorin

关键词:  苯甲酰乌头原碱  芍药苷  抗炎  镇痛

Key words:  benzoylaconine  paeoniflorin  anti-inflammation  analgesia

摘要:

目的 探讨不同剂量苯甲酰乌头原碱配伍芍药苷的抗炎镇痛作用。方法 通过小鼠耳肿胀试验、大鼠足肿胀试验、小鼠热板试验和小鼠扭体试验，研究不同剂量苯甲酰乌头原碱配伍芍药苷的抗炎镇痛作用。结果 苯甲酰乌头原碱配伍芍药苷（高剂量配伍组）可显著抑制二甲苯所致的小鼠耳廓肿胀（P<0.05）。在1 h时，苯甲酰乌头原碱配伍芍药苷可显著抑制大鼠足肿胀（P<0.05或<0.01）；在2 h时，中、高剂量配伍组可显著抑制大鼠足肿胀（P<0.05或<0.01），其中高剂量配伍组相对于芍药苷组和苯甲酰乌头原碱高剂量组，大鼠足肿胀显著减轻（P<0.05）；在4 h时，中、高剂量配伍组可显著抑制大鼠足肿胀（P<0.05或<0.01），高剂量配伍组相对于芍药苷组，大鼠足肿胀显著减轻（P<0.05）。在1 h和1.5 h时，高剂量配伍组可显著升高小鼠热刺激疼痛的痛阈值（P<0.05）。高剂量配伍组可显著升高小鼠冰醋酸刺激疼痛的痛阈值（P<0.05）。苯甲酰乌头原碱配伍芍药苷可显著减少冰醋酸刺激引起的小鼠扭体次数（P<0.01），其中高剂量配伍组相对于芍药苷组和苯甲酰乌头原碱高剂量组，小鼠扭体次数显著减少（P<0.01）。结论 苯甲酰乌头原碱配伍芍药苷可增加苯甲酰乌头原碱、芍药苷的抗炎镇痛作用。

Abstract:

OBJECTIVE To research the anti-inflammation and analgesia effects of different combination therapy with different doses of benzoylaconine and paeoniflorin. METHODS In order to research the anti-inflammatory and analgesic effects of different combination therapy, the xylene induced auricles swelling model, the paws swelling test of rats caused by egg white, the hot-plate test and acetic acid induced writhing model were applied. RESULTS The auricles swelling in mice were remarkably suppressed in combination therapy of benzoylaconine and paeoniflorin(high-dose combination group)(P<0.05). In the paws swelling test of rats caused by egg white, at 1 h, the paws swelling in rats was remarkably inhibited in combination therapy of benzoylaconine and paeoniflorin (P<0.05 or <0.01). At 2 h, the paws swelling in rats was remarkably inhibited in middle-dose benzoylaconine group and high-dose combination group(P<0.05 or <0.01), compared with paeoniflorin group and high-dose benzoylaconine group, the paws swelling in rats was significantly relieved in high-dose combination group(P<0.05). At 4 h, the paws swelling in rats was remarkably inhibited in middle-dose benzoylaconine group and high-dose combination group (P<0.05 or <0.01), compared with paeoniflorin group, the paws swelling in rats was significantly relieved in high-dose combination group(P<0.05). In the hot-plate test, at 1 h and 1.5 h, the pain threshold of mice was markedly elevated in combination therapy of benzoylaconine and paeoniflorin (high-dose combination group)(P<0.05). The pain threshold of mice induced by acetic acid was markedly elevated in (high-dose combination group)(P<0.05). The number of writhing induced by acetic acid in mice was significantly reduced in combination therapy of benzoylaconine and paeoniflorin(P<0.01). Compared with paeoniflorin group and high-dose benzoylaconine group, the number of writhing in mice was remarkably decreased in high-dose combination group(P<0.01). CONCLUSION There are more obvious anti-inflammation and analgesia effect of combination therapy with benzoylaconine and paeoniflorin compared with single therapy with benzoylaconine or paeoniflorin treatment.