石荠苧黄酮提取物的指纹图谱与含量测定分析

Fingerprint Analysis and Determination of Flavonoid Extract from Mosla Scabra

关键词:  石荠苧  黄酮  指纹图谱  含量测定

Key words:  Mosla scabra  flavonoids  fingerprint  determination of content

摘要:

目的 建立石荠苧黄酮提取物的指纹图谱，并测定5个指标成分的含量。方法 采用Hypersil BDS C₁₈色谱柱（250 mm×4.6 mm，5 μm），以甲醇-0.1%磷酸水溶液为流动相，梯度洗脱，流速设为1.0 mL·min^-1，柱温为30 ℃，检测波长270 nm。结果 得到9批次的石荠苧黄酮提取物HPLC指纹图谱，共标识了13个共有峰，其中5个为黄酮类化合物。各样品色谱图与对照色谱图的相似度均>0.96。木犀草苷、木犀草素、芹菜素、山奈酚和7-甲氧基汉黄芩素分别在19.42~194.2 μg·mL^-1、0.520 0~5.200 μg·mL^-1、0.214 0~2.140 μg·mL^-1、0.134 4~1.344 μg·mL^-1和0.013 60~0.136 0 μg·mL^-1内线性关系良好，平均加样回收率分别为100.31%，98.94%，99.15%，100.95%和98.57%，RSD<3%（n=5）。结论 该方法稳定、可靠，可为石荠苧的质量评价提供参考依据。

Abstract:

OBJECTIVE To establish the method of fingerprint analysis and determination of flavonoids from Mosla scabra(MSF) by HPLC. METHODS HPLC separation was carried out on Hypersil BDS C₁₈ (250 mm×4.6 mm, 5 μm) column with a gradient elution consisted of methanol and 0.1% phosphoric acid aqueous solution. The flow rate was kept at 1.0 mL·min^-1. Column temperature was kept constantly at 30℃. The UV absorbance was monitored at 270 nm. RESULTS Nine batches of MSF were collected to generate representative chromatographic fingerprints. Thirteen common peaks were defined in characteristic fingerprints and five selected marker compounds in MSF were well identified by comparing the retention times of the reference standards. The similarity values of 9 samples were all higher than 0.96. Reasonable linear regressions of cynaroside, luteolin, apigenin, kaempferol and 7-methoxy wogonite were obtained in the fingerprints at ranges of 19.42-194.2 μg·mL^-1, 0.520 0-5.200 μg·mL^-1, 0.214 0-2.140 μg·mL^-1, 0.134 4-1.344 μg·mL^-1 and 0.013 60-0.136 0 μg·mL^-1, respectively. Average recovery rates of five compounds were 100.31%, 98.94%, 99.15%, 100.95% and 98.57%, respectively, with the RSD of less than 3% (n=5). CONCLUSION The method is reproducible and precise and can be used for convenient quality assessment of Mosla scabra.