辣椒素对四氯化碳致大鼠急性肝损伤的影响

Influences of Capsaicin on Acute Hepatic Injury Induced by Carborn Tetrachloride in Rats

关键词:  辣椒素  四氯化碳  肝损伤

Key words:  capsaicin  carbon tetrachloride (CCl₄)  liver injury

摘要:

目的 探究辣椒素对四氯化碳所致急性肝损伤的影响。方法 30只SD大鼠随机分为5组：对照组、模型组、辣椒素低、中、高剂量组，每组6只。除对照组外，其他4组大鼠腹腔注射5 ml·kg^-1 25%四氯化碳溶液进行急性肝损伤造模，辣椒素低、中、高剂量组分别给予腹腔注射辣椒素1.0，2.0，5.0 mg·kg^-1，对照组和模型组腹腔注射等量花生油，连续5 d；末次给药1 d后，取大鼠肝脏和血清，并测定各组血清中的丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）和肝组织中的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性及丙二醛（MDA）含量。结果 辣椒素组血清中的ALT和AST显著低于模型组（P<0.05），而CAT酶活性显著高于模型组（P<0.05）；中、高剂量辣椒素组肝组织中的SOD酶活性显著高于模型组（P<0.05），而MDA含量显著低于模型组（P<0.05）；低剂量辣椒素组SOD酶活性也有升高趋势、MDA有降低趋势，但两者与模型组相比没有显著性差异。结论 辣椒素对四氯化碳所致急性肝损伤大鼠具有一定的保护作用，其机制可能与氧化应激有关。

Abstract:

OBJECTIVE To investigate the effect of capsaicin on acute liver injury rats induced by carbon tetrachloride. METHODS Thirty SD rats were randomly divided into five groups:control group, model group, capsaicin low dose, medium dose and high dose group, six rats in each group.In addition to the normal control group, the other four groups were intraperitoneally injected with 5 ml·kg^-1 25% carbon tetrachloride solution for acute liver injury. In the capsaicin low, medium and high dose group were given intraperitoneal injection of capsaicin of 1.0, 2.0, 5.0 mg·kg^-1, blank group and model group were injected with same volume peanut oil for 5 d. After 1 d of the last administration, the liver and serum were taken from the rats, and the serum alanine aminotransferase(ALT), aspartate aminotransferase(AST), superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) were measured. RESULTS The levels of ALT and AST in the serum of the experimental groups were significantly lower than those in the model group(P<0.05), while the activity of CAT was significantly higher than that of the model group(P<0.05). The SOD activity in the middle and high dose capsaicin group were significantly higher than those in the model group(P<0.05), while the MDA content were significantly lower than model group(P<0.05). Compared with the model group, the SOD activity of low dose experiment group also increased, and MDA content decreased, but there was no significant difference. CONCLUSION Capsaicin has a protective effect on SD rats with acute liver injury caused by carbon tetrachloride. The mechanism may be related to oxidative stress.