玫瑰总黄酮对小鼠局灶性脑缺血模型的影响

Effects of Rose Total Flavonoids on Cerebral Ischemia Reperfusion Injury in Mice

关键词:  玫瑰总黄酮  局灶性缺血再灌注  S-100b蛋白

Key words:  rose total flavonoids  focal ischemia-reperfusion  S-100b protein

摘要:

目的 观察玫瑰总黄酮对小鼠局灶性脑缺血再灌注损伤的保护作用。方法 采用线栓法阻塞左侧大脑中动脉，建立小鼠局灶性脑缺血再灌注模型。手术前尼莫地平组、脑络通组、玫瑰总黄酮高中低剂量组分别给予相应的药物灌胃，假手术组和模型组同时给予同体积0.5% CMC灌胃，每天1次，连续给药7 d。末次给药1 h后造模，手术清醒后，对小鼠神经功能进行评分，再灌注22 h，测血清中S-100β蛋白含量，取脑组织TTC染片，计算脑梗死面积。结果 玫瑰总黄酮各剂量组均显著降低小鼠神经功能缺失评分，减少小鼠脑梗死面积，显著降低血清中S-100β蛋白的含量，显著改善脑组织中皮质区的病理损伤。结论 玫瑰总黄酮可较好抑制脑组织缺血再灌注损伤后血清中的S-100β释放，并降低神经功能缺失评分，减少脑梗死面积，改善大脑皮质区的病变情况，对脑缺血再灌注损伤起到一定的保护作用。

Abstract:

OBJECTIVE To observe the protective effects of rose total flavonoids on cerebral ischemia reperfusion injury in mice. METHODS By using the suture block the left middle cerebral artery to establish the focal cerebral ischemia-reperfusion model in mice. Nimodipine group, Nao Luo Tong group, the high, medium and low dose of rose total flavonoids groups were given related drugs via intragastric administration before the operation. The sham operation group, and ischemia-reperfusion group were given the same volume of 0.5% sodium carboxymethyl cellulose for seven days, once a day. One hour after the last administration model, after operation sober, evaluating neural function score of mice, reperime 22 h, measuring the concentration of S-100β in serum, TTC stained of brain tissue slices, calculating the area of cerebral infarction. RESULTS Rose total flavonoids groups significantly decreased the nerve function score in mice, reduced the area of cerebral infarction in mice, significantly decreased the content of S-100β protein in serum, significantly improved the pathological changes of cortical areas in brain tissue. CONCLUSION Rose total flavonoids could inhibit S-100β in serum released after cerebral ischemia reperfusion injury, improve the nerve injury symptoms, reduce the infraction area of brain and improve the pathological changes of cerebral cortex, thereby rose total flavonoids play a protective role on cerebral ischemia reperfusion injury.