HPLC同时测定蒙药材黄花铁线莲中4种黄酮含量

Simultaneous Determination of Four Kinds of Flavonoids in Clematis Intricata Bunge of Mongolian Medicinal Herb by HPLC

关键词:  黄花铁线莲  蒙药材  木犀草苷  金丝桃苷  芦丁  槲皮素  含量测定  高效液相色谱法

Key words:  Clematis intricata Bunge  Mongolian medicinal herb  luteoloside  hyperoside  rutin  quercetin  content determination  HPLC

摘要:

目的 通过对蒙药材黄花铁线莲中4种黄酮的HPLC含量测定，建立黄花铁线莲质量控制方法。方法 采用YMC C₁₈色谱柱（4.6 mm×250 mm，5 μm），甲醇-0.04%磷酸水溶液为流动相，梯度洗脱，检测波长350 nm，柱温25℃，流速1.0 mL·min^-1。结果 木犀草苷、金丝桃苷、芦丁和槲皮素分别在0.021 37~0.427 4，0.010 85~0.217 0，0.021 41~0.428 2，0.017 46~0.349 2 μg内与峰面积线性关系良好；精密度、重复性、稳定性的RSD均< 2%，平均回收率为97.4%~102.3%。结论 该方法简便、准确、可靠，重复性好，可用于蒙药材黄花铁线莲的质量评价与控制。

Abstract:

OBJECTIVE To establish a method to control the quality of Clematis intricata Bunge through the content determination of the four kinds of flavonoids in Clematis intricata Bunge of Mongolian medicinal herb. METHODS The HPLC analysis was carried out on YMC C₁₈ column(4.6 mm×250 mm, 5 μm) with a methanol-0.04% phosphoric acid solution as the mobile phase for gradient elution at a flow rate of 1.0 mL·min^-1. The detection wavelength was 350 nm, and the column temperature was 25℃. RESULTS Luteoloside, hyperoside, rutin and quercetin exhibited good linear relationships within the ranges of 0.021 37-0.427 4, 0.010 85-0.217 0, 0.021 41-0.428 2, 0.017 46-0.349 2 μg, respectively. The RSD of precision, repeatability and stability tests were <2%, the average recoveries were ranged in 97.4%-102.3%. CONCLUSION The method is simple, accurate and reliable with food repeatability, which can be used for the quality evaluation and control of Clematis intricata Bunge of Mongolian crued drugs.