升麻药材及其颗粒的HPLC指纹图谱质量控制研究

Study on the HPLC Fingerprint Chromatogram of Cimicifugae Rhizoma and Cimicifuga Species Root Formula Granules

关键词:  升麻药材  升麻颗粒  HPLC  指纹图谱

Key words:  Cimicifugae Rhizoma  Cimicifuga Species Root formula granules  HPLC  fingerprint chromatogram

摘要:

目的 建立升麻药材及其颗粒的HPLC指纹图谱，为升麻药材及其颗粒的质量控制以及工艺评价提供依据。方法 采用C₁₈色谱柱，以乙腈-0.1%磷酸溶液为流动相梯度洗脱，流速1.0 mL·min^-1，检测波长254 nm，柱温30 ℃。结果 所建立的HPLC指纹图谱能够对升麻药材及其颗粒各组分进行良好分离，具有较好的精密度、重复性和稳定性；并对图谱中的6个主峰进行了化学指认。结论 采用HPLC建立的指纹图谱方法简单，重复性好，能够准确分析升麻药材及其颗粒的物质组成以及有效物质群的变化，可用于升麻药材及其颗粒的质量控制和工艺评价。

Abstract:

OBJECTIVE To establish the HPLC fingerprint chromatogram of Cimicifugae Rhizoma and Cimicifuga Species Root formula granules, so as to provide basis for quality control and process evaluation of Cimicifugae Rhizoma and Cimicifuga Species Root formula granules. METHODS HPLC was performed on C₁₈ column with the mobile phase consisting of acetonitrile and 0.1% phosphoric acid solution in gradient mode. The flow rate was 1.0 mL·min^-1. Under the temperature of 30℃, with a detection wavelength of 254 nm. RESULTS The components of Cimicifugae Rhizoma and Cimicifuga Species Root formula granules could be separated well using HPLC. The developed method was precise, reproducible and stable. And 6 main peak of the chromatogram were chemical composition identified. CONCLUSION The established method is simple, accurate and reproducible in the components determination of Cimicifugae Rhizoma and Cimicifuga Species Root formula granules, which can be used for the quality control and process evaluation.