响应面法优化雪松松针中金丝桃苷的回流提取工艺

Optimization of Reflux Extraction Technology of Hyperin in Pine Needle of Cedrus Deodra by Response Surface Methodology

关键词:  雪松松针  金丝桃苷  回流提取工艺  Box-Behnken设计  响应面法

Key words:  pine needle of Cedrus deodra  hyperin  reflux extraction technology  Box-Behnken design  response surface methodology

摘要:

目的 优化雪松松针中金丝桃苷的回流提取工艺。方法 采用HPLC测定雪松松针中金丝桃苷的含量；基于单因素试验结果，以金丝桃苷含量为指标，根据Box-Behnken设计对影响回流提取的3个因素（料液比、乙醇浓度、提取时间）进行考察；通过响应面分析优化雪松松针中金丝桃苷的最佳回流提取工艺。结果 优化得到的最佳回流提取工艺参数为乙醇浓度60%、料液比1︰16（g·mL^-1）、回流提取1次，时间为100 min；在此工艺条件下提取得到的金丝桃苷平均含量为0.119 6 mg·g^-1，与预测值0.119 4 mg·g^-1的相对偏差较小。结论 优选得到的雪松松针中金丝桃苷回流提取工艺合理、可行。

Abstract:

OBJECTIVE To optimize the reflux extraction technology of hyperin in pine needle of Cedrus deodra. METHODS The content of hyperoside in pine needle of Cedrus deodara was determined by HPLC. On the basis of single factor test, the hyperoside content was used as indexes, three factors (solid-to-liquid ratio, ethanol concentration and extraction time) were studied by Box-Behnken design, the reflux extraction technology of hyperin in pine needle of Cedrus deodra was optimized by response surface methodology. RESULTS The optimal parameters of reflux extraction technology were as follows:ethanol concentration was 60%, solid-to-liquid ratio was 1:16(g·mL^-1), extraction time was 100 min for once. Under the above conditions, the average content of hyperin was 0.119 6 mg·g^-1, which was similar to the predicted value 0.119 4 mg·g^-1. CONCLUSION The preferred extraction technology of hyperin in pine needle of Cedrus deodra is reasonable and feasible.