新橙皮苷大鼠生育力与早期胚胎发育毒性研究

Fertility and Early Embryonic Developmental Toxicity Study of Neohesperidin in Rats

关键词:  新橙皮苷  生殖毒性  生育力  早期胚胎发育

Key words:  neohesperidin  reproduction toxicity  fertility  early embryonic development

摘要:

目的 评价新橙皮苷对大鼠生育力及早期胚胎发育的毒性。方法 200只大鼠（♀♂各半）根据性别和体质量采用分层随机法分成对照（0.5%羧甲基纤维素钠）组、环磷酰胺（20 mg·kg^-1）组、新橙皮苷低、中、高（0.45，0.9和1.8 mg·kg^-1）组。雄鼠于交配前4周开始给药至交配期结束。雌鼠于交配前2周开始给药至妊娠第6天。实验期间每周测定动物体质量。交配结束后，雄性动物对附睾尾精子进行精子检查；妊娠第15天，对雌性动物子宫内容物包括黄体、着床腺、活胎、死胎及吸收胎、胎盘等进行检查。结果 给药期间，各组别动物一般观察均未见明显异常。与对照组相比，各给药组雄鼠交配前体质量、脏器重量、精子计数及精子活力数值未见显著性差异（P>0.05）；对照组和新橙皮苷3个剂量组精子总畸形率均在2%以内。新橙皮苷各给药组雌鼠体质量、脏器重量、黄体数、着床腺、活胎数、吸收胎数、死胎数与对照组相比均无显著性差异（P>0.05）。病理组织学检查结果显示，雄鼠睾丸、附睾、前列腺和精囊腺未见给药相关改变，对照组和新橙皮苷组雌鼠子宫和阴道可见明显孕后变化。结论 新橙皮苷对大鼠生育力及早期胚胎发育未见明显毒性。

Abstract:

OBJECTIVE To evaluate fertility and early embryonic developmental toxicity of neohesperidin in rats. METHODS According to sex and bodyweight, 200 rats(100 males and 100 females) were stratified randomly assigned to 5 groups, as control group (0.5% sodium carboxymethyl cellulose solution), cyclophosphamide group (20 mg·kg^-1), neohesperidin low, middle and high dose group (0.45, 0.9 and 1.8 mg·kg^-1). Male rats were administrated since 4 weeks before mating period to the end of mating period. Female rats were administrated since 2 weeks before mating period to 6th gestation period. At termination, sperm from the epididymides cauda was collected for examination. At 15th gestation period, uterine contents were examined as corpora luteum, implantation site, viable fetuses, deaths fetuses, absorptive fetus and placenta. RESULTS During administration, no abnormal behavior was observed for each group. No significant difference was found between control group and other groups for male rats on premating bodyweight, organ weight, enumeration of sperm reserves and sperm motility (P>0.05). Rate of total deformation was under 2% for control group and neohesperidin groups. No significant difference found between control group and neohesperidin groups for female rats on bodyweight, organ weight, corpora luteum, implantation site, viable fetuses, deaths fetuses, absorptive fetus (P>0.05). The result of histopathology examination showed that there was no pathological change related to dose noted for testis, epididymis, prostate and seminal vesicle; obvious pregnancy changes were found on uterus and vagina for control group and neohesperidin group. CONCLUSION There is no toxicity found for the fertility and early embryonic developmental toxicity of neohesperidin in rats.