UHPLC特征图谱结合多组分含量评价首乌藤饮片的质量

Quality Evaluation of Polygoni Multiflori Caulis by UHPLC Specific Chromatogramand Multi-Component Quantitative Analysis

关键词:  首乌藤  特征图谱  聚类分析(CA)  主成分分析(PCA)  质量评价

Key words:  Polygoni Multiflori Caulis  specific chromatogram  principle component analysis(PCA)  cluster analysis(CA)  quality evaluation

摘要:

目的 建立首乌藤的UHPLC特征图谱，测定2，3，5，4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷、大黄素和大黄素甲醚的含量。结合聚类分析法（CA）和主成分分析法（PCA）对首乌藤饮片的质量进行研究。方法 色谱柱：Shiseido C₁₈（2.1 mm×100 mm，2.7 μm）；流动相：乙腈-0.1%磷酸溶液梯度洗脱；流速：0.3 mL·min^-1；柱温：30℃；检测波长：254 nm。运用SPSS 20.0软件进行PCA和CA分析。结果 特征图谱确立6个特征峰，40批首乌藤的相似度为0.655~0.998；2，3，5，4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷、大黄素及大黄素甲醚含量分别为0.01~1.96，0.02~3.15和0.01~1.48 mg·g^-1。PCA和CA结果将首乌藤饮片分为3类，反映了不同批次首乌藤饮片的质量特征。结论 UHPLC特征图谱和多组分含量结合PCA和CA可以对首乌藤饮片的质量进行客观、有效的评价。

Abstract:

OBJECTIVE To establish the UHPLC specific chromatogram and determine 2,3,5,4-tetrahydroxyl diphenylethylene-2-O-β-D-glucoside, rheum emodinand rheochrysidin of Polygoni Multiflori Caulis. To evaluate the quality of Polygoni Multiflori Caulis based on principal component analysis(PCA) and cluster analysis(CA). METHODS UHPLC analysis was performed on a shiseido C₁₈ column(2.1 mm×100 mm, 2.7 μm). The mobile phase was composed of 0.1% phosphoric acid and acetonitrile with gradient elution. And the flow rate was 0.3 mL·min^-1, the detection wavelength was 254 nm. The SPSS 20.0 software was used for PCA and CA analysis. RESULTS The method for specific chromatogram was established six characteristic peaks. The similarities of 40 batches of Polygoni Multiflori Caulis were 0.655-0.998. The content of 2,3,5,4-tetrahydroxyl diphenylethylene-2-O-β-D-glucoside, rheum emodin, rheochrysidin in different batches ranged from 0.01 to 1.96 mg·g^-1, from 0.02 to 3.15 mg·g^-1, and from 0.01 to 1.48 mg·g^-1. PCA and CA results showed that all of Polygoni Multiflori Caulis were classified into 3 categories, and reflected the quality characteristics of different batches. CONCLUSION The UHPLC specific chromatogram coupled with PCA and CA can objectively and effectively assess the quality of Polygoni Multiflori Caulis.