ASE-HPLC测定仙茅中仙茅苷的含量及其指纹图谱研究

Contents Determination of Curculigoside and Fingerprint Chromatograms of Curculiginis Rhizoma by ASE-HPLC

关键词:  仙茅  指纹图谱  快速溶剂萃取  仙茅苷  正交试验

Key words:  Curculigins Rhizoma  fingerprint  accelerated solvent extraction  curculigoside  orthogonal design

摘要:

目的 获得仙茅药材的HPLC指纹图谱，同时研究快速萃取仙茅中仙茅苷及其含量测定的方法，为其质量控制提供依据。方法 采用正交试验优选ASE 350快速溶剂萃取系统的最佳提取方法，采用HPLC测定仙茅苷的量，色谱柱为Thermo Syncronis C₁₈（100 mm×3 mm，3 μm），流动相为乙腈-0.1%磷酸水溶液，梯度洗脱，体积流量为0.5 mL·min^-1，检测波长为285 nm，柱温为40℃。指纹图谱共有模式采用国家药典委员会中药色谱指纹图谱相似度评价系统（2.0版）进行处理分析。结果 采用萃取温度100℃、静态萃取时间5 min、循环提取2次的快速提取方法最优。在指纹图谱研究中，标定了15个共有峰，建立了对照指纹图谱，20批药材与共有模式之间相似性良好，相似度均>0.9。结论 本方法快速、准确，可用于仙茅药材的综合质量评价。

Abstract:

OBJECTIVE To establish the HPLC fingerprint and study on rapid extraction and content determination of curculigoside in Curculiginis Rhizoma, provide evidence for quality control. METHODS The orthogonal design was used for ASE 350 condition optimization, and the content of curculigoside was determined by HPLC, using Thermo Syncronis C₁₈(100 mm×3 mm, 3 μm) column, acetonitrile-0.1% phosphoric acid as mobile phase by gradient elution, the flow rate was 0.5 mL·min^-1, the column temperature was 40℃, detection wavelength was 285 nm. The data analysis was performed with Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (Version 2.0). RESULTS The optimal extraction conditions were extracting two times at 100℃ and static extraction time was 5 min. There were fifteen common peaks in the fingerprint. The similarity of fingerprints of 20 batches of Curculiginis Rhizoma were all >0.9. CONCLUSION The method is rapid, accurate, and can be used to control quality evaluation for Curculigins Rhizoma.