新疆不同产区塔里木马鹿鹿茸对比研究

Comparative study on different regions velvet antlers of Cervus Elaphus yarkandensis in Xinjiang

关键词:  塔里木马鹿  鹿茸多肽  成骨细胞  碱性磷酸酶

Key words:  cervus elaphus yarkandensis  antler peptides  osteoblasts  ALP

摘要:

目的 检测新疆5个不同产地塔里木亚种马鹿鹿茸质量性状、多肽（42，26，15 kDa）含量及其对小鼠前成骨细胞增殖和活性的影响。方法 对比分析不同产地鹿茸性状（直径、干鲜比、茸长、眉枝、水分、总灰分和浸出物）；采用BCA蛋白定量试剂盒测定鹿茸多肽中的蛋白含量并作聚类分析，采用SDS-PAGE电泳法鉴别鹿茸多肽类型；建立小鼠成骨细胞模型，采用MTT及ELISA法分析其对细胞增殖和功能的作用。结果 不同产地塔里木鹿茸药材中鹿茸质量性状存在差异，塔里木河沙雅县鹿茸质量性状较好，鹿茸多肽含量高，显著提高了小鼠成骨细胞增殖率40.74%及碱性磷酸酶活性。结论 新疆沙雅地区塔里木亚种马鹿鹿茸活性较好。本实验为建立塔里木马鹿亚种鹿茸质量标准的提升提供了数据支撑。

Abstract:

OBJECTIVE To investigate the quality, contents and effects of antler peptides (42, 26, 15 kDa) in Cervus elaphus yarkandensis from different regions. METHODS Characters (diameters, DW/FW, length, brow tine, ash content and extractum) of velvet antlers from different regions were comparative researched. Concentrations of different antler peptides were measured by BCA protein assay kit, and data was analyzed by clustering analysis. The SDS-PAGE method was used to detect types of antler peptides. Moreover, by mouse osteoblasts model, cell proliferations and cell activity were detected by MTT and ELISA method. RESULTS Qualities of velvet antler in Cervus elaphus yarkandensis were significantly different from different locations. Among these velvet antlers, them had the best characteristics and the highest content of antler peptides which harvesting in Xayar County, what's more, the proliferation (40.74%) and ALP secretion of mouse osteoblast was significant increased in Xayar County antler peptide group. CONCLUSION The Xayar County antler had the best characteristics. It provides data supporting for raising the quality standard of Cervus elaphus yarkandensis antler peptides.