| 引用本文: | 李大伟,吴永江.中压制备色谱法制备高纯度石杉碱甲[J].中国现代应用药学,2018,35(1):63-66. |
| LI Dawei,WU Yongjiang.Separation and Preparation of high-purity huperzine A by medium pressure preparative chromatography[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(1):63-66. |
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| 摘要: |
| 目的 采用中压制备色谱法从蛇足石杉浸膏中制备高纯度石杉碱甲。方法 分别使用聚合物反相填料和C18反相填料为分离介质，串联两步中压制备色谱，从蛇足石杉浸膏中分离石杉碱甲单体。结果 制备的石杉碱甲单体纯度为99.7%，总体回收率达到87.5%。结论 该法利用聚合物填料和C18填料的各自特点，通过合理的工艺衔接，为获得医药级石杉碱甲提供了一条高效、绿色和经济的途径。 |
| 关键词: 蛇足石杉 石杉碱甲 中压制备色谱 聚合物填料 |
| DOI：10.13748/j.cnki.issn1007-7693.2018.01.014 |
| 分类号:R284.2 |
| 基金项目:国家自然科学基金项目（81173021） |
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| Separation and Preparation of high-purity huperzine A by medium pressure preparative chromatography |
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LI Dawei, WU Yongjiang
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College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China
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| Abstract: |
| OBJECTIVE To establish a method for the preparation of high-purity huperzine A from the extract of huperzia serrata. METHODS By using the polymer reversed phase medium and C18 reversed phase medium and two steps of medium pressure preparation in series, huperzine A monomer was isolated and prepared from the huperzia serrata extract. RESULTS The purity of 99.7% huperzine A monomer was obtained, and the overall recovery rate was 87.5%. CONCLUSION This method utilizes the characteristics of polymer medium and C18 medium to provide an efficient, green and economical way to obtain pharmaceutical grade huperzine A through technological linkage. |
| Key words: huperzia serrata huperzine A medium pressure preparative chromatography polymer medium |
