不同产地锁阳药材HPLC指纹图谱研究及2种黄酮成分含量测定

HPLC Fingerprints Analysis of Cynomorium Songaricum Rupr. from Different Aeas and Content Determination of Two Flavonoids

关键词:  锁阳  指纹图谱  不同产地  儿茶素  根皮苷  聚类分析  主成分分析

Key words:  Cynomorium songaricum Rupr.  fingerprint  different areas  catechin  phloridzin  CA  PCA

摘要:

目的 建立不同产地锁阳药材HPLC指纹图谱，并测定其中2种黄酮成分的含量。方法 采用HPLC-DAD技术，以Dikma Spursil C₁₈色谱柱（4.6 mm×250 mm，5 μm），甲醇-0.2%甲酸溶液为流动相梯度洗脱，建立锁阳药材的指纹图谱并进行含量测定；采用中药指纹图谱相似度评价系统（2012版）对12批样品进行共有峰确认及相似度评价；通过SPSS 21.0统计软件采用聚类分析（CA）和主成分分析（PCA）对HPLC指纹图谱进行模式识别研究。结果 建立了锁阳药材指纹图谱，12批锁阳药材的相似度均>0.90；确定共有峰22个，并对其中儿茶素、根皮苷含量进行测定，其含量均值分别为0.320，0.057 mg·g^-1。CA将不同批次锁阳药材分为4类，反映了12个不同产区锁阳药材的质量特征；通过PCA筛选出累计贡献率达到89.349%的5个主成分，得到决定锁阳药材质量的4个化学成分。结论 建立的HPLC指纹图谱结合含量测定、CA、PCA方法可以客观、全面、有效地用于锁阳药材的质量评价。

Abstract:

OBJECTIVE To establish the HPLC fingerprints of Cynomorium songaricum Rupr (CSR) from different areas, and determine the content of two flavonoids. METHODS HPLC-DAD method were developed to establish fingerprints for CSR, and determine the contents, performanced on the Dikma Spursil C₁₈(4.6 mm×250 mm, 5 μm) and methanol-0.2% formic acid water as the mobile phase for gradient elution. Similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (2012 edition) was used to confirm the common peaks in 12 batches of samples and evaluate the similarity. SPSS 21.0 statistical software was used to make cluster analysis(CA) and principal component analysis(PCA) for the HPLC fingerprint. RESULTS The common mode for CSR fingerprint was established, the similarity was >0.90 in 12 batches of CSR, and 22 common fingerprint peaks were identified. And the contents of catechin and phloridzin were determined, the average of contents were 0.320, 0.057 mg·g^-1, respectively. Different batches of samples could be classified into four groups by using CA, reflecting the quality characteristics of 12 batches of CSR in different areas. Five principal components with a cumulative contribution rate of 89.349% were screened by using PCA to obtain four chemical compositions that could determine the quality of CSR. CONCLUSION The HPLC fingerprint combined with content determination, CA, PCA methods can objectively, effectively, fully for quality evaluation of CSR.