基于高通量测序的青叶胆转录组研究

Analysis of the Transcriptome of Swertia Mileensis based on High-throughput Sequencing

关键词:  青叶胆  转录组  Illumina Hiseq 2500  獐牙菜苦苷

Key words:  Swertia mileensis  transcriptome  Illumina Hiseq 2500  swertiamain

摘要:

目的 探讨青叶胆中獐牙菜苦苷生物合成的遗传基础。方法 采用Illumina Hiseq 2500高通量测序技术，对青叶胆全草进行转录组测序。结果 共获得68 643个unigene，平均长度901 bp，共有42 954条unigene注释到NR，SwissProt，COG，KOG，GO，Pfam数据库中，獐牙菜苦苷生物合成的3个阶段中，有43条unigene参与中间体的生成，3条unigene参与萜类骨架合成，25条unigene可能参与萜类最后的修饰。结论 首次对青叶胆转录组进行测序分析，获得了可能参与獐牙菜苦苷生物合成的候选基因，为后续功能基因的挖掘奠定了基础。

Abstract:

OBJECTIVE To explore the genetic basis of the biosynthesis of the swertiamarinin in S. mileensis. METHODS The high throughput sequencing by Illumina Hiseq 2500 was used to sequence its transcriptome of S. mileensis. RESULTS A total of 685 643 unigenes were assembled with an average length of 901 bp. Among them, 42 954 unigenes were annotated in the public databases NR, SwissProt, COG, KOG, GO and Pfam. The results showed that 43 unigenes were involved in the production of intermediate, 3 unigenes catalyze synthesis of terpenoids skeleton, and 25 unigenes might participate in terpene post-modification in the three steps of biosynthesis of swertiamain. CONCLUSION This study is the first comprehensive study on transcriptome of S. mileensis, and candidate genes involves in the biosynthesis of swertiamarin are obtained. The transcriptome data constitutes a much more abudant genetic resource that can be utilized to benefit further molecular biology studies on S. mileensis.