| 引用本文: | 刘姝静,刘玉强,戚晓杰,刘育含,吕新,孙建之,才谦.基于HPLC特征图谱和主要成分含量测定的龙胆药材干燥方法研究[J].中国现代应用药学,2018,35(7):1012-1015. |
| LIU Shujing,LIU Yuqiang,QI Xiaojie,LIU Yuhan,LYU Xin,SUN Jianzhi,CAI Qian.Study on Drying Methods of Gentiana Scabra Bge. Based on HPLC Characteristic Fingerprints and Content Determination of Main Components[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(7):1012-1015. |
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| 摘要: |
| 目的 通过测定龙胆药材不同干燥方法所得样品的HPLC特征图谱及3种主要成分的含量，确定龙胆药材的最佳干燥方法。方法 采用Welchrom C18色谱柱（4.6 mm×250 mm，5 μm）；流动相：甲醇-水，梯度洗脱；流速：1.0 mL·min-1；进样量：10 μL；检测波长：240 nm；柱温：25℃。结果 建立了龙胆药材鲜品及不同方法干燥后样品的HPLC特征图谱，同时测定了样品中3种主要成分的含量。结论 龙胆药材的最佳干燥方法为阴干法。 |
| 关键词: 龙胆 干燥方法 特征图谱 含量测定 龙胆苦苷 獐牙菜苦苷 獐牙菜苷 |
| DOI：10.13748/j.cnki.issn1007-7693.2018.07.015 |
| 分类号:R284.1 |
| 基金项目:国家中药标准化项目（ZYBZH-Y-TJ-43） |
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| Study on Drying Methods of Gentiana Scabra Bge. Based on HPLC Characteristic Fingerprints and Content Determination of Main Components |
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LIU Shujing, LIU Yuqiang, QI Xiaojie, LIU Yuhan, LYU Xin, SUN Jianzhi, CAI Qian
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College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China
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| Abstract: |
| OBJECTIVE To confirm the optimal drying method of Gentiana scabra Bge. based on HPLC characteristic fingerprint and contents determination of three main components in the samples. METHODS The condition was as followes:Welchrom C18 column(4.6 mm×250 mm,5 μm); mobile phase:methanol-water with gradient elution; flow rate:1 mL·min-1; injection volume:10 μL; detection wavelength:240 nm; column temperature:25℃. RESULTS The HPLC characteristic fingerprint of Gentiana scabra Bge. was established, while the contents of 3 main components in samples of fresh and different drying methods were determined. CONCLUSION The shade drying is the optimal drying method of Gentiana scabra Bge. |
| Key words: Gentiana scabra Bge. drying methods characteristic fingerprint content determination gentiopicroside swertiamarin sweroside |
