阿尔泰锦鸡儿不同药用部位主要黄酮苷的含量测定

Study on Content Determination of Main Flavonoid Glycosides in Different Medical Parts of Caragana Altaica Kom. Pojark

关键词:  阿尔泰锦鸡儿  不同药用部位  黄酮苷  芦丁  山奈酚-3-O-芸香糖苷  含量测定

Key words:  Caragana altaica kom.pojark  different medical parts  flavonoid glycosides  rutin  kaempferol-3-O-rutinoside  content determination

摘要:

目的 测定采集于新疆阿勒泰地区的10批次阿尔泰锦鸡儿根、茎、叶、花与果实药材中芦丁和山奈酚-3-O-芸香糖苷的含量。方法 色谱条件：Inertsil ODS-3色谱柱（250 mm×4.6 mm，5 μm），检测波长254 nm，流速1.0 mL·min^-1，进样量5 μL，柱温25℃，流动相为0.2%磷酸（A）-乙腈（B），线性梯度洗脱（0~5 min，10%→14% B；5~10 min，14%→17% B；10~16 min，17%→20% B；16~21 min，20%→22% B；21~30 min，22%→25% B；30~40 min，25% B）。结果 10批次阿尔泰锦鸡儿叶、花与果实药材中芦丁的平均含量为：果实6.83 mg·g^-1，叶6.16 mg·g^-1，花3.58 mg·g^-1；山奈酚-3-O-芸香糖苷的平均含量为：果实1.48 mg·g^-1，叶2.44 mg·g^-1，花1.01 mg·g^-1。根与茎供试品溶液中均未检测到这2种黄酮苷成分。结论 建立的方法简便、稳定性、重复性良好，为阿尔泰锦鸡儿药材黄酮类成分的研究提供了实验基础，亦为建立该药材的质量标准提供了重要的实验数据。

Abstract:

OBJECTIVE To determine the content of rutin and kaempferol-3-O-rutinoside in the roots, stems, leaves, flowers and fruits of Caragana altaica kom. pojark from Xinjiang Altai Region.METHODS HPLC conditions:Inertsil ODS-3 column(250 mm×4.6 mm, 5 μm), detection wavelength at 254 nm, flow rate at 1.0 mL·min^-1, injection volume was 5 μL and column temperature was 25℃. A gradient elution of 0.2% phosphoric acid (A) and acetonitrile (B) solution were performed with following procedure:0-5 min, 10%→14% B; 5-10 min, 14%→17% B; 10-16 min, 17%→20% B; 16-21 min, 20%→22% B; 21-30 min, 22%→25% B; 30-40 min, 25% B.RESULTS The average content of rutin in 10 batches of fruits, leaves and flowers was 6.83, 6.16, 3.58 mg·g^-1 and the average content of kaempferol-3-O-rutinoside was 1.48, 2.44, 1.01 mg·g^-1, respectively. However, neither rutin nor kaempferol-3-O-rutinoside were detected in the roots and stems of Caragana altaica kom. pojark.CONCLUSION The content determination method established in this study is simple and showes good stability and repeatability. Furthermore, this study provides experimental basis for the study of flavonoid glycosides in Caragana altaica kom. pojark. and provides essential experimental data for the establishment of quality standard of that plant.