| 引用本文: | 辛文秀,钟里科,张轶雯,方罗,郑小卫,丁海樱,黄萍.小春花药材质量标准研究[J].中国现代应用药学,2018,35(4):542-546. |
| Xin Wenxiu,Zhong Like,Zhang Yiwen,Fang Luo,Zheng Xiaowei,Ding Haiying,Huang Ping.Study on Quality Standards for Sceptridium Ternatum[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(4):542-546. |
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| 小春花药材质量标准研究 |
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辛文秀,钟里科,张轶雯,方罗,郑小卫,丁海樱,黄萍
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1.浙江省肿瘤医院药剂科, 杭州 310022;2.浙江省头颈肿瘤转化医学研究重点实验室, 杭州 310022
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| 摘要: |
| 目的 制定并考察小春花药材质量标准，为进一步开发小春花药材提供基础。方法 采用紫外-可见分光光度法建立小春花中总黄酮的含量测定方法；采用HPLC同时测定小春花药材中槲皮素和山奈酚的含量。结果 建立小春花总黄酮的测定方法，槲皮素为在30.23~181.40 μg·mL-1呈良好线性关系，R2=0.998 4；确定药材中槲皮素、山奈酚的含量测定方法，槲皮素在2.64~21.12μg·mL-1呈良好的线性关系，R2=0.999 8，山奈酚在2.00~16.00μg·mL-1呈良好的线性关系，R2=0.999 9；小春花总黄酮以槲皮素计不得<0.2‰，山奈酚计不得<0.6‰。结论 本研究对小春花药材进行质量控制，提升并建立了新的小春花药材质量标准。 |
| 关键词: 小春花 总黄酮 槲皮素 山奈酚 质量标准 |
| DOI：10.13748/j.cnki.issn1007-7693.2018.04.018 |
| 分类号:R284.1;R914.101 |
| 基金项目:浙江省中医药科技计划项目（2017ZB022）；浙江省中医药重点项目（2016ZZ009）；2016浙江省151第二层次培养项目；2016浙江省卫生创新人材培养项目 |
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| Study on Quality Standards for Sceptridium Ternatum |
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Xin Wenxiu1,2,3, Zhong Like1, Zhang Yiwen1, Fang Luo1, Zheng Xiaowei1, Ding Haiying1, Huang Ping1,2,3
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1.Department of Pharmacy, Zhejiang Cancer Hospital, Hangzhou 310022, China;2.Key Laboratory of Head & amp;3.Neck Cancer Translational Research of Zhejiang Province, Hangzhou 310022, China
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| Abstract: |
| OBJECTIVE To establish the quality standards for Sceptridium ternatum, and lay the foundation for its further development. METHODS The content of total flavonoids was assayed by UV-Vis spectrophotometry. The contents of quercetin and kaempferol were determined by HPLC. RESULTS There was a good linear relationship between the absorbance values and the quercetin concentration when it was in the range of 30.23-181.40 μg·mL-1(R2=0.998 4). The quercetin and kaempferol were in good linear relationships in the range of 2.64-21.12 μg·mL-1(R2=0.999 8) and 2.00-16.00 μg·mL-1(R2=0.999 9), respectively. The quercetin/kaempferol content in Sceptridium ternatum should not be less than 0.2‰ and 0.6‰, respectively. CONCLUSION The quality control standard for Sceptridium ternatum is modified and a new one is provided to guarantee the quality of raw medical materials. |
| Key words: Sceptridium ternatum total flavonoids quercetin kaempferol quality standards |
