郁金散对抗生素相关性大肠湿热证大鼠肠黏膜屏障损伤的修复作用

Effect of Yujin Power on Intestinal Mucosal Barrier of Antibiotic Related Large Intestine Dampness-heat Syndrome Rats Model

关键词:  郁金散  抗生素相关性大肠湿热证  炎症因子  菌群失调  肠黏膜屏障

Key words:  yujin power  antibiotic related large intestine dampness-heat syndrome(A-DHS)  inflammatory factors  flora imbalance  intestinal mucosal barrier

摘要:

目的 探讨郁金散对抗生素相关性大肠湿热证大鼠肠黏膜屏障损伤的修复作用。方法 采用复合因素诱导建立抗生素相关性大肠湿热证大鼠模型，随机分为正常组、模型组、阳性组（柳氮磺吡啶）、郁金散高、中、低剂量组，每组10只，连续给药7 d，定期观测各组大鼠的一般状态、体质量、摄食量、肛温，对肠道敏感性和粪便性状进行评分，测定粪便含水量及血清TNF-α、IL-1β、二胺氧化酶（DAO）、D-乳酸含量，检测肠黏膜SIgA及结肠组织髓过氧化物（MPO）含量，计数结肠内容物中乳酸杆菌、双歧杆菌、肠球菌、大肠杆菌，分析结肠组织形态变化。结果 与模型组比较，给予郁金散治疗后，高、中剂量组大鼠体质量和摄食量明显增加，肛温降低，肠道敏感性减小，4 h内排便次数、粪便性状评分、粪便含水量均有所减少，血清TNF-α、IL-1β、DAO、D-乳酸及结肠组织MPO含量均减小，肠黏膜SIgA含量升高，肠内容物中乳酸杆菌、双歧杆菌显著增加，而肠球菌、大肠杆菌明显减少，HPS评分显著减小。结论 郁金散对抗生素相关性大肠湿热证大鼠肠黏膜屏障损伤具有一定的的修复作用，其作用机制与抑制血清TNF-α、IL-1β、DAO、D-乳酸和结肠组织MPO含量的增加以及肠黏膜SIgA含量的减少，调节肠道菌群向正常水平恢复有关。

Abstract:

OBJECTIVE To research the influence of Yujin power on intestinal mucosal barrier in the rats model of antibiotic related large intestine dampness-heat syndrome. METHODS To establish the rats model of antibiotic related large intestine dampness-heat syndrome induced by internal and external factors. Sixty SD rats were randomly divided into the normal control group, the model group, positive control group (Sulfasalazine), high, middle and low dose of Yujin power groups, 10 rats in each group. The treatment lasted for 7 d. Body weights, food intake, anal temperature of all the rats were determined. Abdominal with drawal reflex and the parting grade of faeces shape were scored. The defecation frequency and stool water content were measured. The contents of TNF-α, IL-1β, DAO, D-lactate in serum, the SIgA content in colon mucosa and the MPO content in colon homogenates were assessed by radioimmunoassay. The number of Lactobacillus, Bifidobacterium, Enterococcus and Escherichia coli in the colon content of rats was measured and counted. The intestinal morphology was evaluated by histological examination. RESULTS Compared with the model group, after treatment with Yujin power for 7 d, all the indexes were recovered and high-dose of Yujin Power group performed the best and followed by middle-dose group. The rats body weight and food intake were increased, and anal temperature was decreased. The score of abdominal with drawal reflex and the parting grade of faeces shape were decreased, and the defecation frequency and stool water content were also decreased. The contents of TNF-α, IL-1β, DAO, D-lactate in serum, the MPO content in colon homogenates were decreased, but the SIgA content in colon mucosa was increased. The numbers of Lactobacillus, Bifidobacterium were increased, but the number of Enterococcus and Escherichia coli in the colon content of rats were decreased. Consequently, the HPS score was decreased as well. CONCLUSION Yujin power can reduce the intestinal mucosa injury in rats with antibiotic related large intestine dampness-heat syndrome. The mechanism can be related to inhibition against the excessive secretion of TNF-α, IL-1β, DAO, D-lactate in serum and the MPO content in colon homogenates or the less secretion SIgA content in colon mucosa, regulating intestinal flora to normal level.