HPLC测定沅江产芦笋中3种酚酸和6种黄酮类成分含量

Quantitative Determination of Three Phenolic Acids and Six Flavonoids of Phragmites Communis (Cav.) Trin. ex Steud from Yuanjiang by HPLC

关键词:  芦笋  酚酸  黄酮  高效液相色谱法

Key words:  Phragmites communis(Cav.) Trin.ex Steud  phenolic acids  flavonoids  HPLC

摘要:

目的 建立同时测定沅江产芦笋中原儿茶酸、绿原酸、香草酸、柚皮苷、芦丁、槲皮素、木犀草素、山奈酚、芹菜素含量的HPLC。方法 采用Diamonsil C₁₈色谱柱（250 mm×4.6 mm，5 μm），以甲醇-0.1%甲酸水溶液为流动相，梯度洗脱，双波长检测（酚酸：283，327 nm；黄酮：283，335 nm），流速1 mL·min^-1，柱温35℃，进样量为10 μL。结果 3种酚酸及6种黄酮分别在25 min和50 min内分离，线性范围为14.8~500 μg·mL^-1（r=0.999 0~0.999 7），检测限0.006 3~0.053 9 μg·mL^-1，定量限0.021 1~0.179 7 μg·mL^-1，加样回收率为98.73%~102.44%，RSD为1.27%~2.20%（n=6）。结论 该方法准确灵敏、重复性好，可用于检测芦笋中9种成分含量。

Abstract:

OBJECTIVE To establish quantitative determination method of the protocatechuic acid, chlorogenic acid, vanillic acid, naringin, rutin, quercetin, luteolin, kaempferol and apigenin in Phragmites communis (Cav.) Trin. ex Steud from Yuanjiang by HPLC. METHODS The chromatographic separation was performed on a Diamonsil C₁₈ column (250 mm×4.6 mm, 5 μm) kept at 35℃, using methanol-0.1% formic acid aqueous as the mobile phase at a flow rate of 1 mL·min^-1 through gradient elution, and the detection wavelength was set at dual wavelength (phenolic acids:283, 327 nm; flavonoids:283, 335 nm), the injection volume was 10 μL. RESULTS The three phenolic acids and six flavonoids were isolated at 25 min and 50 min, the method exhibited linear range of 14.8-500 μg·mL^-1 (r=0.999 0-0.999 7), the limits of detection (LOD) was 0.006 3- 0.053 9 μg·mL^-1, the limits of quantification (LOQ) was 0.021 1-0.179 7 μg·mL^-1 and the recovery rate was 98.73%-102.44%, RSD was 1.27%-2.20% (n=6). CONCLUSION The method is accurate, sensitive, reproducible, and can be used to detect and quantitative the nine components in Phragmites communis (Cav.) Trin. ex Steud.