| 引用本文: | 李永春,李晓兰,赵美荣,赵艳,张智.响应面法优化椿皮中铁屎米-6-酮生物碱超声辅助提取工艺[J].中国现代应用药学,2018,35(5):628-632. |
| LI Yongchun,LI Xiaolan,ZHAO Meirong,ZHAO Yan,ZHANG Zhi.Optimization of Ultrasound-assisted Extraction Technology of Canthin-6-one from Bark of Ailanthus Altissima by Response Surface Methodology[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(5):628-632. |
|
| 摘要: |
| 目的 应用响应面法优化超声辅助提取椿皮中铁屎米-6-酮生物碱的工艺条件。方法 以铁屎米-6-酮生物碱提取量为指标，在单因素考察乙醇提取铁屎米-6-酮的基础上，采用中心组合试验设计和响应面分析法优选提取工艺参数。结果 最佳提取工艺条件为乙醇浓度68%，液固比32 mL·g-1，超声温度54℃，超声时间38 min，铁屎米-6-酮生物碱提取量为26.19 μg·g-1，接近于模型预测值（26.31 μg·g-1）。结论 优选的超声提取工艺合理可靠，可为臭椿的开发应用提供实验依据。 |
| 关键词: 椿皮 铁屎米-6-酮 超声辅助提取 响应面法 |
| DOI：10.13748/j.cnki.issn1007-7693.2018.05.002 |
| 分类号:R284.1 |
| 基金项目:国家自然科学基金项目（31400459） |
|
| Optimization of Ultrasound-assisted Extraction Technology of Canthin-6-one from Bark of Ailanthus Altissima by Response Surface Methodology |
|
LI Yongchun1,2, LI Xiaolan2, ZHAO Meirong2, ZHAO Yan2, ZHANG Zhi1
|
|
1.College of Forestry, Northeast Forestry University, Harbin 150040, China;2.College of Life Science, Chifeng University, Chifeng 024000, China
|
| Abstract: |
| OBJECTIVE To optimize ultrasonic-assisted extraction technology of canthin-6-one from bark of Ailanthus altissima.METHODS With yield of canthin-6-one as index, extraction technology parameters were optimized by central composite design-response surface methodology on the basis of the single factor investigation of conditions for extracting canthin-6-one by ethanol.RESULTS Optimum extraction conditions were as follows:ethanol concentration 68%, liquid-solid ratio 32 mL·g-1, ultrasound temperature 54℃, extraction time 38 min. Under these conditions, yield of canthin-6-one was 26.19 μg·g-1, which was close to the predicted value(26.31 μg·g-1).CONCLUSION This optimized technology is reasonable and reliable to provide a basis for development and application of Ailanthus altissima. |
| Key words: bark of Ailanthus altissima canthin-6-one ultrasound-assisted extraction response surface methodology |
