HPLC双波长法同时测定炎热清片中6种有效成分的含量

Simultaneous Determination of Six Components in Yanreqing Tablets by Double-wavelength HPLC

关键词:  炎热清片  龙胆苦苷  栀子苷  芒果苷  黄芩苷  黄芩素  汉黄芩素  高效液相色谱法

Key words:  Yanreqing tablets  gentiopicroside  geniposide  mangiferin  baicalin  baicalein  wogonin  HPLC

摘要:

目的 建立HPLC双波长法同时测定炎热清片中龙胆苦苷、栀子苷、芒果苷、黄芩苷、黄芩素和汉黄芩素6种成分的含量。方法 采用Agilent TC-C₁₈色谱柱（4.6 mm×250 mm，5 μm），流动相为甲醇∶0.2%磷酸水溶液（梯度洗脱），柱温为35℃，流速为1.0 mL·min^-1，检测波长0~20 min为254 nm，同时检测龙胆苦苷、栀子苷、芒果苷，20~50 min为280 nm，同时检测黄芩苷、黄芩素、汉黄芩素。结果 龙胆苦苷、栀子苷、芒果苷、黄芩苷、黄芩素、汉黄芩素分别在18.92~189.2 ng（r=0.999 9），107.44~1 074.4 ng（r=0.999 9），9.82~98.2 ng（r=0.999 9），767.68~7 676.8 ng（r=1.000 0），100.94~1 009.4 ng（r=0.999 9），49.84~498.4 ng（r=0.999 9）线性良好，平均回收率（n=9）分别为98.31%，98.35%，98.40%，98.03%，98.46%，98.24%，RSD分别为0.4%，0.3%，0.3%，0.4%，0.4%，0.3%。结论 该方法灵敏、简便、准确，可用于炎热清片的质量控制。

Abstract:

OBJECTIVE To establish a new double-wavelength HPLC method for simultaneous determination of 6 main components in Yanreqing tablets. METHODS The method was performed on an Agilent TC-C₁₈ column (4.6 mm×250 mm, 5 μm). The mobile phase was consisited of methanol-0.2% phosphoric acid by gradient elution at 35℃ column temperature. The flow rate was 1.0 mL·min^-1. The detection wavelength was 254 nm for gentiopicroside, geniposide, mangiferin at 0-20 min, and 280 nm for baicalin, baicalein, wogonin at 20-50 min. RESULTS Gentiopicroside, geniposide, mangiferin, baicalin, baicalein, wogonin showed a good linear relationship in the range of 18.92-189.2 ng (r=0.999 9), 107.44-1 074.4 ng (r=0.999 9), 9.82-98.2 ng (r=0.999 9), 767.68-7 676.8 ng (r=1.000 0), 100.94-1 009.4 ng (r=0.999 9), 49.84-498.4 ng (r=0.999 9), respectively. The average recoveries were 98.31%, 98.35%, 98.40%, 98.03%, 98.46%, 98.24%, the RSD were 0.4%, 0.3%, 0.3%, 0.4%, 0.4%, 0.3%. CONCLUSION The method is sensitive, simple, accurate, and suitable for the quality control of Yanreqing tablets.