金荞麦提取物体外抗流感病毒作用研究

Study on the Anti-influenza Virus of Extracts from Fagopyrum Dibotrys (D. Don) Hara in Vitro

关键词:  金荞麦  总黄酮  流感病毒  鸡胚

Key words:  Fagopyrum dibotrys(D. Don) Hara  total flavonoids  influenza virus  chicken embryo

摘要:

目的 研究金荞麦提取物的体外抗流感病毒作用。方法 采用血凝试验，考察金荞麦提取物对鸡胚内流感病毒增殖的抑制作用；采用MTT法检测细胞活性，考察金荞麦提取物对流感感染细胞活性的影响。结果 与病毒对照组比较，金荞麦提取物高剂量组（10 mg·mL^-1）、中剂量组（5 mg·mL^-1）均能显著降低鸡胚尿囊液的血凝滴度（P<0.01），而低剂量组（2.5 mg·mL^-1）则无显著性差异；在0.63~2.50 mg·mL^-1浓度内，金荞麦提取物各剂量组流感感染细胞的活性均明显高于病毒对照组，组间均存在显著性差异（P<0.01），且病毒抑制率随着药物浓度的增大而增高，呈一定的量效关系，其中金荞麦提取物最大无毒浓度（2.5 mg·mL^-1）对流感病毒的直接抑制率达66.12%。结论 金荞麦提取物具有一定的体外抗流感病毒作用。

Abstract:

OBJECTIVE To explore the anti-influenza virus of extracts from Fagopyrum dibotrys in vitro. METHODS Hemagglutination titer test was used to investigate the inhibitory effect of extracts from Fagopyrum dibotrys on the proliferation of influenza virus in chicken embryo. The cytoactive was tested by MTT assay and investigated the effect of extracts from Fagopyrum dibotrys on the activity of infected cells by influenza virus. RESULTS Compared with the virus control group, the high dose group(10 mg·mL^-1) and the middle dose group(5 mg·mL^-1) of extracts from Fagopyrum dibotrys significantly decreased the hemagglutination titer of chicken embryo allantoic fluid(P<0.01), whlie the low dose group(2.5 mg·mL^-1) was no significant difference. In the concentration range of 0.63-2.50 mg·mL^-1, the cytoactive of the groups of extracts from Fagopyrum dibotrys were significantly higher than those in the virus control group(P<0.01), and the inhibition rate increased in a dose-dependent. The inhibition rate of maximum non-toxic concentration of extracts from Fagopyrum dibotrys(2.5 mg·mL^-1) was 66.12%. CONCLUSION The extracts from Fagopyrum dibotrys has anti-influenza effect in vitro.