浙产6种威灵仙类药材中齐墩果酸的含量测定

Determination of Oleanolic Acid in Six Species of Clematis Raw Drugs from Zhejiang Province

关键词:  威灵仙类药材  齐墩果酸  高效液相色谱法  含量测定

Key words:  Clematis  oleanolic acid  HPLC  content determination

摘要:

目的 采用HPLC测定浙产6种威灵仙类药材中齐墩果酸的含量，为扩大浙江地区威灵仙药源提供参考。方法 采用Hypersil BOS C₁₈色谱柱（4.6 mm×150 mm，5 μm），流动相为甲醇-水（90∶ 10），流速为0.8 mL·min^-1，柱温为25℃，检测波长为210 nm。结果 威灵仙、扬子铁线莲、粗齿铁线莲、毛蕊铁线莲、柱果铁线莲中齐墩果酸含量分别为0.381%，0.172%，0.395%，0.001 84%，1.70%，山木通中齐墩果酸含量太低无法计算。结论 柱果铁线莲、粗齿铁线莲可考虑作为威灵仙的替代资源，其他品种能否作为威灵仙入药，尚有待探讨。

Abstract:

OBJECTIVE To determine contents of oleanolic acid in six species of clematis raw drugs from Zhejiang province, so as to provide a reference for medicinal resources expansion from Zhejiang province. METHODS Hypersil BOS C₁₈ column (4.6 mm×150 mm, 5 μm) was used at 25℃, methanol-water (90:10) was used as the mobile phase, the flow rate was 0.8 mL·min^-1, the detection wavelength was 210 nm. RESULTS The contents of oleanofic acid in Clematis chinensis, C. ganpiniana, C. argentilucida, C. lasiandra and C. uncinata were 0.381%, 0.172%, 0.395%, 0.001 84%, 1.70%, while its content was too low to calculated in C. finetiana. CONCLUSION C. uncinata and C. argentilucida can be considered as alternative resources of medicinal clematis, but whether other species is suitable as medicine remains shall to be explored.