美洲大蠊提取物对肝硬化门静脉高压大鼠NF-κB和MIF表达的影响

Effect of Extract of Periplaneta Americana on NF-κB and MIF's Expression in Rats with Portal Hypertension

关键词:  康复新液  普萘洛尔  门静脉高压  核转录因子-κB  巨噬细胞移动抑制因子

Key words:  extract of periplaneta americana  propranolol  portal hypertension  nuclear transcription factor-κB(NF-κB)  macrophage migration inhibitory factor(MIF)

摘要:

目的 观察康复新液（extract of Periplaneta americana，APA）对门静脉高压症大鼠核转录因子-κB（nuclear transcriptionfactor-κB，NF-κB）及巨噬细胞移动抑制因子（macrophage migration inhibitory factor，MIF）表达的影响。方法 60只SD大鼠分为正常对照组、模型组、普萘洛尔组和APA高、中、低剂量组，每组10只。采用CCl₄加乙醇建立大鼠门静脉高压症模型，边造模边给药8周。测各组大鼠门静脉压力，同时观察肝脏组织形态学改变，并用免疫组化方法检测肝脏中NF-κB及MIF的表达水平。结果 模型组门静脉压力较正常对照组明显升高（P<0.05），APA组及普萘洛尔组门静脉压力较模型组明显下降（P<0.05），但仍高于正常组（P<0.05）；APA高、中剂量组不仅可以改善受损肝脏的病理形态；还可降低肝脏组织中NF-κB、MIF的表达。结论 APA不仅可以改善门静脉系统的高动力状态，其治疗效果优于普萘洛尔，而且还具有改善肝硬化门静脉高压症中肝脏组织形态的作用，其机制除了与其具有促进血管增生、改善血液循环、促进组织修复有关之外，还可能与其抑制肝组织中NF-κB、MIF的表达有关。

Abstract:

OBJECTIVE To investigate the effects of extract of Periplaneta americana(APA) on nuclear transcription factor-κB(NF-κB) and macrophage migration inhibitory factor(MIF)'s expression in rats with portal hypertension. METHODS Sixty SD rats were divided into normal control group, model group, propranolol group, APA high, medium and low dose groups, 10 rats in each group. The model of rats with portal hypertension were prepared with CCl₄ and alcohol, 8 weeks of administration while modeling. The portal vein pressure of the rats were measured by direct manometric method, and the liver tissue morphology changes were observed. Besides, the expression level of NF-κB and MIF were detected by immunohistochemistry. RESULTS The portal vein pressure of model group was increased significantly compared with that of normal group(P<0.05), while such index in treatment groups(APA groups and propranolol group) was higher than that of normal group(P<0.05), but was lower significantly than that of model group(P<0.05). APA high and medium dose groups not only improved the pathology of damaged liver, but also decreased the expression of NF-κB and MIF in liver tissue. CONCLUSION APA can not only alleviate the hyperdynamic state of portal system, it is superior to the propranolol group, but also can decrease the expression of NF-κB and MIF, and finally improve the symptoms of portal hypertension. In addition to promoting angiogenesis, improving blood circulation and promoting tissue repair, the mechanism may be related to the inhibition of the expression of NF-κB and MIF in liver tissue.