单细胞基因组和转录组的共测定方法研究进展

Progress in Parallel Sequencing of the Genomes and Transcriptomes of Single Cells

关键词:  多手性中心  手性分析  高效液相色谱法  毛细管电泳

Key words:  multichiral centers  chiral analysis  HPLC  capillary electrophoresis

摘要:

单细胞测序提供了大量样品测序所不能提供的异质性等信息。然而由于细胞生长发育受多种因素调节，仅依靠基因型或者表型信息无法全面、准确地说明问题。因此，开发单细胞基因组转录组共测定技术为研究基因变异与表型变异间的关系提供了强有力的工具。目前，单细胞基因组与转录组共测定技术主要分为全细胞裂解法（如：G&T-Seq、DR-Seq），细胞膜裂解法（如：SIDR、微流控芯片法）以及其他方法（如：Simul-Seq、Gel-Seq）。本文对这几类技术进行了简要介绍，并对其前景进行了展望。

Abstract:

Single-cell sequencing provides information, such as heterogeneity, that is omitted by bulk sample sequencing. However, since cell growth and development are regulated by many factors, relying on genotype or phenotype information alone is not able to comprehensively and accurately explain the problem. Co-detection of single-cell DNA-Seq and RNA-Seq offers a strong tool for researchers on the aspect of the relationship between gene variations and phenotype variations. Up to present, there are strategies described below have been employed:whole cell lysis (e.g.:G&T-Seq, DR-Seq), plasma membrane lysis (e.g.:SIDR, microfluidics platform method) and others (e.g.:Simul-Seq, Gel-Seq). This review will introduce and compare these methods in addition to the prediction of their research trends.