C57BL/6J糖尿病小鼠模型的优化研究

Optimization of C57BL/6J Diabetic Mice Model

关键词:  糖尿病模型  小鼠  链脲佐菌素  C57BL/6J小鼠  模型优选

Key words:  diabetic model  mice  streptozotocin  C57BL/6J mice  model optimization

摘要:

目的 优化链脲佐菌素（streptozotocin，STZ）诱导的C57BL/6J糖尿病小鼠模型。方法 采用单用不同剂量STZ（180 mg·kg^-1单次给药和275 mg·kg^-1均分5次，每次55 mg·kg^-1，连续5 d）或4周高脂饮食联合不同剂量STZ（50 mg·kg^-1单次给药；100 mg·kg^-1单次给药；150 mg·kg^-1单次给药；200 mg·kg^-1均分2次给药，间隔72 h），建立糖尿病小鼠模型，检测各组小鼠空腹血糖、体质量、日饮水量及日进食量，比较各组造模成功率及稳定性。结果 STZ 180 mg·kg^-1单次给药、高脂饮食4周+STZ 150 mg·kg^-1单次给药、高脂饮食4周+STZ 200 mg·kg^-1均分2次给药得到的糖尿病小鼠模型，其高血糖的持续时间较长且稳定。结论 STZ 180 mg·kg^-1单次给药是较为理想的1型糖尿病模型；4周高脂饮食联合STZ 150 mg·kg^-1单次给药或200 mg·kg^-1均分2次给药均是较为理想的2型糖尿病模型。

Abstract:

OBJECTIVE To optimize the C57BL/6J diabetic mice model induced by streptozotocin(STZ). METHODS Different diabetic mice model were established with different doses of STZ intraperitoneal administration (180 mg·kg^-1 single dose and 275 mg·kg^-1 averaged by 5 d, 55 mg·kg^-1 each day) and 4-week high-fat diet combined with different doses of STZ(50 mg·kg^-1 single dose, 100 mg·kg^-1 single dose, 150 mg·kg^-1 single dose and 200 mg·kg^-1 for two times at 72 h intervals). Fasting blood glucose, weight, water-drinking and food-consumption were determined, the modeling rate and stability of each group were compared. RESULTS Diabetic model induced by STZ 180 mg·kg^-1 single dose, four weeks high-fat diet combined with STZ 150 mg·kg^-1 single dose or 200 mg·kg^-1 for two times administration were more stable in the long run. CONCLUSION STZ 180 mg·kg^-1 single dose is a suitable type 1 diabetic model, meanwhile, four weeks high-fat diet combined with STZ 150 mg·kg^-1 single dose or 200 mg·kg^-1 for two times administration are appropriate type 2 diabetic model.