GC测定克痢痧胶囊中的冰片、甲基丁香酚和丁香酚的含量

Determination of Borneol, Methyleugenol and Eugenol in Kelisha Capsules by GC

关键词:  气相色谱法  克痢痧胶囊  冰片  甲基丁香酚  丁香酚

Key words:  GC  Kelisha capsules  borneol  methyleugenol  eugenol

摘要:

目的 采用GC测定克痢痧胶囊中的冰片、甲基丁香酚和丁香酚的含量。方法 采用GC直接进样，色谱柱为AgilentHP-INNOWAX毛细管柱，检测器为氢火焰离子化检测器，温度为250℃，采用程序升温，进样口温度为210℃；流速为1.2 mL·min^-1，分流比为15︰1，进样量为1 μL。结果 冰片、甲基丁香酚和丁香酚的线性范围分别是48.59~728.88 μg·mL^-1（r=0.999）；4.29~64.39 μg·mL^-1（r=0.999）和31.83~477.48 μg·mL^-1（r=0.999），精密度、重复性和稳定性试验的RSD均≤2.17%，平均加样回收率分别是98.69%（RSD=0.3%），93.10%（RSD=1.6%）和99.41%（RSD=0.5%）。结论 本方法操作简单、快速、准确性和重复性好，可用于克痢痧胶囊的质量控制。

Abstract:

OBJECTIVE To establish a method for determination of borneol, methyleugenol and eugenol in Kelisha capsules by GC. METHODS Using the direct injection by GC, the column was Agilent HP-INNOWAX capillary column, FID was used as detector with a temperature of 250℃, and the inlet temperature was 210℃, using the temperature program. The flow rate was 1.2 mL·min^-1, split ratio was 15︰1 and the volume was 1 μL. RESULTS Borneol, methyleugenol and eugenol were separated with a good linearity in the concentration of 48.59-728.88 μg·mL^-1 (r=0.999) for borneol, 4.29-64.39 μg·mL^-1 (r=0.999) for methyleugenol, and 31.83-477.48 μg·mL^-1 (r=0.999) for eugenol. RSDs of precision, stability and reproducibility tests were ≤ 2.17%. The average recoveries for borneol, methyleugenol and eugenol were 98.69% (RSD=0.3%), 93.10% (RSD=1.6%) and 99.41% (RSD=0.5%). CONCLUSION The method is simple, accurate and reproducible, and can be used in the quality control of Kelisha capsules.