GC同时测定藏药七味铁屑丸中4种物质的含量

Simultaneous Determination of Four Substances in Tibetan Medicine of Qiwei Tiexue Pill by GC

关键词:  藏药  土木香内酯  异土木香内酯  去氢木香内酯  木香烃内酯  七味铁屑丸  气相色谱法

Key words:  Tibetan medicine  alantolactone  isoalantolactone  dehydrocostuslactone  costunolide  Qiwei Tiexue pill  GC

摘要:

目的 建立同时测定藏药七味铁屑丸中土木香内酯、异土木香内酯、去氢木香内酯和木香烃内酯的GC法。方法 HP-5毛细管色谱柱（30 m×0.32 mm，0.25 μm）；FID检测器；柱温箱温度140℃，进样口温度220℃，检测器温度250℃；载气为氮气，流速为1.0 mL·min^-1，空气流速450 mL·min^-1，氢气流速45 mL·min^-1；进样量1 μL；分流比20︰1。结果 土木香内酯、异土木香内酯、去氢木香内酯和木香烃内酯的线性范围分别为7.120 2~71.202 2 μg·mL^-1（r=0.998 4）；5.478 5~54.785 1 μg·mL^-1（r=0.997 1）；8.195 3~98.344 1 μg·mL^-1（r=0.996 7）；6.395 4~76.744 8 μg·mL^-1（r=0.997 2）；平均回收率分别为98.11%（RSD=1.04%），98.12%（RSD=1.09%），98.39%（RSD=1.26%），97.52%（RSD=1.25%）。结论 本方法可有效可靠地对七味铁屑丸中藏木香和木香进行定量控制。

Abstract:

OBJECTIVE To estahlish a method for the simultaneous determination of alantolactone, isoalantolactone, dehydrocostuslactone and costunolide in Tibetan medicine Qiwei Tiexue pill by GC. METHODS Samples of Qiwei Tiexue pill were determined by HP-5 capillary column(30 m×0.32 mm, 0.25 μm). The column oven temperature was 140℃. FID was used as detector with a temperature of 250℃, and the inlet temperature was 220℃. The carries gas was nitrogen and the flow rate of carries gas was 1.0 mL·min^-1. The air flow rate was 450 mL·min^-1. The hydrogen flow rate was 45 mL·min^-1. The injection volume was 1 μL. Split ratio was 20:1. RESULTS The linear ranges of alantolactone, isoalantolactone, dehydrocostuslactone and costunolide were 7.120 2-71.202 2 μg·mL^-1(r=0.998 4); 5.478 5-54.785 1 μg·mL^-1(r=0.997 1); 8.195 3-98.344 1 μg·mL^-1 (r=0.996 7); 6.395 4-76.744 8 μg·mL^-1(r=0.997 2), with average recoveries(n=6) of 98.11%(RSD=1.04%), 98.12%(RSD=1.09%), 98.39%(RSD=1.26%), 97.52%(RSD=1.25%), respectively. CONCLUSION This method is effective and reliable, which can be used for quality control for the determination of Inula racemosa and Aucklandia lapp in Qiwei Tiexue pill.