青蒿琥酯自微乳对NAFLD大鼠的保护作用及机制研究

Protective Effect and Mechanism of Artesunate Self-microemulsions on NAFLD Rats

关键词:  青蒿琥酯  自微乳  非酒精性脂肪肝

Key words:  artesunate  self-microemulsions  non-alcoholic fatty liver disease (NAFLD)

摘要:

目的 探讨青蒿琥酯自微乳对非酒精性脂肪肝（non-alcoholic fatty liver disease，NAFLD）模型大鼠的保护作用及作用机制。方法 高脂饲料诱导10周建立大鼠NAFLD模型。SD大鼠分为7组：正常组，模型组，青蒿琥酯组，多烯磷脂酰胆碱组，青蒿琥酯自微乳高、中、低剂量组。给药10周后处死所有动物，检测各组大鼠血清ALT、AST、TG、TC、TNF-α和IL-10指标水平。结果 与模型组比较，各药物治疗组（除低剂量组外）均能明显改善血清ALT、AST、TG、TC、TNF-α和IL-10指标水平（P<0.05或<0.01）。结论 青蒿琥酯自微乳通过调节脂质代谢、抑制炎性细胞因子的释放，减轻肝细胞的损伤，从而对高脂饮食诱导的NAFLD模型大鼠具有一定的保护作用。

Abstract:

OBJECTIVE To study the protective effect of the artesunate self-microemulsions on rats with non-alcoholic fatty liver disease (NAFLD). METHODS The model of NAFLD was constructed by feeding rats with high fat diet for 10 weeks. SD rats were randomly divided into the normal group, the model group, the essentinale group, the artesunate group, the high, middle and low dose of artesunate self-microemulsions group. All the rats were sacrificed after 10 weeks of treatment. Serum sample were collected for the contents detection of ALT, AST, TG, TC, TNF-α and IL-10. RESULTS Compared with the model group, each drug treatment group (except low dose of artesunate self-microemulsions group) could significantly reduce ALT, AST, TG, TC, TNF-α and IL-10 levels in serum (P<0.05 or <0.01). CONCLUSION The artesunate self-microemulsions has a better protective effect on NAFLD rats induced by high fat diet. The mechanisms may be related to inhibiting lipid peroxide, reducing the level of inflammatory mediator in the liver.