| 引用本文: | 唐晓雷,何慧楠,张思雨,王德慧,陈昊媛,卢姝言,李雪惠,赵大庆,刘莉,齐滨.柱前衍生-HPLC测定不同等级的梅花鹿鹿茸饮片中氨基酸的含量[J].中国现代应用药学,2019,36(6):650-654. |
| TANG Xiaolei,HE Huinan,ZHANG Siyu,WANG Dehui,CHEN Haoyuan,LU Shuyan,LI Xuehui,ZHAO Daqing,LIU Li,QI Bin.Determination of Amino Acids in Different Grades of Antler Pieces from Cervus Nippon by Pre-column Derivatization-HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(6):650-654. |
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| 柱前衍生-HPLC测定不同等级的梅花鹿鹿茸饮片中氨基酸的含量 |
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唐晓雷1, 何慧楠2, 张思雨1, 王德慧1, 陈昊媛1, 卢姝言1, 李雪惠2, 赵大庆2, 刘莉1, 齐滨1
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1.长春中医药大学药学院, 长春 130117;2.长春中医药大学吉林省人参科学研究院, 长春 130117
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| 摘要: |
| 目的 建立柱前衍生-HPLC测定不同等级梅花鹿鹿茸饮片中17种氨基酸成分含量的方法，并比较氨基酸含量的差异。方法 采用HPLC，以异硫氰酸苯酯进行柱前衍生，梯度洗脱的方法，检测不同等级梅花鹿鹿茸饮片中17种氨基酸的含量。结果 不同等级的梅花鹿鹿茸饮片的总氨基酸成分含量差异显著。氨基酸含量蜡片 > 粉片 > 纱片 > 骨片。结论 梅花鹿鹿茸饮片的质量受采割部位的影响。该方法适用于梅花鹿鹿茸饮片中氨基酸成分的含量测定，可为其等级的制定提供理论依据。 |
| 关键词: 梅花鹿 鹿茸饮片 氨基酸含量 柱前衍生-高效液相色谱法 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.06.002 |
| 分类号:R284.1 |
| 基金项目:国家中药标准化项目（ZYBZH-Y-JL-26，ZYBZH-C-JL-22） |
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| Determination of Amino Acids in Different Grades of Antler Pieces from Cervus Nippon by Pre-column Derivatization-HPLC |
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TANG Xiaolei1, HE Huinan2, ZHANG Siyu1, WANG Dehui1, CHEN Haoyuan1, LU Shuyan1, LI Xuehui2, ZHAO Daqing2, LIU Li1, QI Bin1
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1.Changchun University of Chinese Medicine, College of Pharmacy, Jilin Ginseng Academy, Changchun 130117, China;2.Changchun University of Chinese Medicine, Jilin Ginseng Academy, Changchun 130117, China
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| Abstract: |
| OBJECTIVE To establish a method for the determination of 17 amino acids contents in different grades of antler pieces from Cervus nippon using pre-column derivatization-HPLC, and to compare the difference of amino acid contents. METHODS The contents of 17 kinds of amino acids in different grades of antler pieces were detected by HPLC with pre-column derivatization and gradient elution with phenyl isothiocyanate. RESULTS There were significant differences in the total amino acid contents of different grades of antler pieces. Amino acid contents from high to low was wax, powder, sand and bone chips. CONCLUSION The quality of antler pieces is affected by the position of harvest. The method is suitable for the determination of the amino acid contents in the antler pieces, which can provide a theoretical basis for the formulation of antler pieces. |
| Key words: Cervus nippon antler pieces amino acid content pre-column derivatization-HPLC |
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