灰毡毛忍冬不同发育期花蕾中3种成分含量的变化研究

Content Comparison of Three Components in Floral Buds at Different Developmental Stages of Lonicera Macranthoides Hand. -Mazz.

关键词:  灰毡毛忍冬  花蕾  绿原酸  灰毡毛忍冬皂苷乙  川续断皂苷乙  高效液相色谱-紫外吸收检测-蒸发光散射检测法  采收

Key words:  Lonicera macranthoides Hand.-Mazz.  bud  chlorogenic acid  macranthoidin B  dipsacoside B  HPLC-UV-ELSD  harvest

摘要:

目的 比较灰毡毛忍冬不同发育期花蕾中绿原酸、灰毡毛忍冬皂苷乙和川续断皂苷乙的含量。方法 色谱柱：CAPCELL PAK C₁₈ MG（250 mm×4.60 mm，5 μm）；流动相：乙腈（A）-0.4%醋酸溶液（B），梯度洗脱；流速：1.0 mL·min^-1；紫外检测波长：330 nm；蒸发光散射检测器条件：漂移管温度110℃，气体流速3.0 L·min^-1，撞击器关；柱温：30℃。收集不同发育期（幼蕾期、三青期、大白期、银花期、金花期、凋花期）花蕾样品，采用HPLC-UV-ELSD串联技术，以外标两点法计算绿原酸的含量，以外标两点法对数方程计算灰毡毛忍冬皂苷乙和川续断皂苷乙的含量。结果 灰毡毛忍冬中绿原酸和川续断皂苷乙含量以三青期最高，分别为6.934%，1.800%；灰毡毛忍冬皂苷乙以幼蕾期最高，为9.351%，较三青期高0.227%。结论 通过对灰毡毛忍冬花蕾不同发育期的含量比较可见，最佳采收期为三青期，此时绿原酸、灰毡毛忍冬皂苷乙和川续断皂苷乙含量高。

Abstract:

OBJECTIVE To compare the content differences of chlorogenic acid, macranthoidin B and dipsacoside B in floral buds at different developmental stages of Lonicera macranthoides Hand. -Mazz. METHODS The chromatographic column was CAPCELL PAK C₁₈ MG (250 mm×4.60 mm, 5 μm); the moblie phase was acetonitrile (A)-0.4% acetic acid (B) with gradient elution; flow rate was 1.0 mL·min^-1; UV detection wavelength was 330 nm; ELSD testing condition was as follows:tube temperature 110℃, gas flow 3.0 L·min^-1 and impactor off; column temperature was 30℃. Using HPLC-UV-ELSD series technology, the contents of three components in floral buds collected at different developmental stages (youlei stage, sanqing stage, dabai stage, yinhua stage, jinhua stage, diaohua stage) in Lonicera macranthoides Hand. -Mazz were measured. The content of chlorogenic acid was calculated by outside the two point method, and the content of macranthoidin B and dipsacoside B was calculated by outside the two point logarithmic equation method. RESULTS The contents of chlorogenic acid and dipsacoside B were 6.934% and 1.800%, respectively, were the highest at sanqing stage in Lonicera macranthoides Hand. -Mazz.. However, the highest content of macranthoidin B was 9.351% at youlei stage, which was 0.227% higher than that at sanqing stage. CONCLUSION By comparing the contents of chlorogenic acid, macranthoidin B and dipsacoside B in floral buds at different developmental stages in Lonicera macranthoides Hand. -Mazz., sanqing period is the best harvest time with high content.