| 引用本文: | 王海英,杨继,刘新,张键泰,朱国元.石松的化学成分研究[J].中国现代应用药学,2018,35(12):1859-1864. |
| WANG Haiying,YANG Ji,LIU Xin,ZHANG Jiantai,ZHU Guoyuan.Chemical Constituents Study of Lycopodium Japonicum[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(12):1859-1864. |
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| 摘要: |
| 目的 研究石松全草的化学成分。方法 石松全草采用80%的乙醇回流提取，乙醇提取物经过酸溶碱沉后，用乙酸乙酯进行萃取，乙酸乙酯相经过硅胶柱层析、中压制备液相色谱及HPLC等进行分离纯化，采用现代波谱技术结合理化性质鉴定化合物结构。结果 从石松乙醇提取物中分离并鉴定了11个化合物，包括8个生物碱：α-异玉柏碱（1）、α-玉柏碱（2）、去-N-甲基-α-玉柏碱（3）、石松灵碱（4）、lycoposerramine M（5）、玉柏宁碱（6）、lyconadin D（7）、石松佛利星碱（8）；2个三萜类化合物：石松三醇（9），α-芒柄花萜醇（10）；1个酚苷类化合物：4-glucosyloxy-3-methoxyphenl trans-propenoicethyl ester（11）。结论 化合物1为新化合物，命名为α-异玉柏碱（α-isoobscurine）。化合物7和11为首次从该植物中分离得到。 |
| 关键词: 石松 伸筋草 生物碱 三萜 酚苷 |
| DOI：10.13748/j.cnki.issn1007-7693.2018.12.021 |
| 分类号:R284.1 |
| 基金项目:澳门科学技术发展基金资助项目(033/2015/A1) |
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| Chemical Constituents Study of Lycopodium Japonicum |
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WANG Haiying1, YANG Ji2, LIU Xin2, ZHANG Jiantai2, ZHU Guoyuan2
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1.Taizhou Municipal Hospital, Taizhou 318000, China;2.State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau 999078, China
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| Abstract: |
| OBJECTIVE To investigate the chemical constituents from the whole plant of Lycopodium japonicum. METHODS The whole plant of L. japonicum was refluxed with 80% ethanol. The concentrated extract was dissolved in 1% HCl solution. The supernatant was then basified and partitioned with EtOAc. The EtOAc fraction was chromatographed on silica gel column, purified by MPLC and preparative HPLC. The structures of isolated compounds were elucidated on the basis of spectroscopic data analyses. RESULTS Eleven compounds were isolated from the ethanolic extract of L. japonicum, including eight alkaloids:α-isoobscurine (1), α-obscurine (2), des-N-methyl-α-obscurine (3), lycodoline (4), lycoposerramine M (5), obscurinine (6), lyconadin D (7), and lycoflexine (8); two triterpenoids:lycoclavanol (9), α-onocerin (10); and a phenolic glycoside:4-glucosyloxy-3-methoxyphenyl trans-propenoic ethyl ester (11). CONCLUSION Compound 1 is a new compound and named α-isoobscurine. Compounds 7 and 11 were isolated from this plant for the first time. |
| Key words: Lycopodium japonicum Shenjincao alkaloids triterpenoids phenolic glycoside |
