金蝉花水提物对高糖诱导的肾小管上皮细胞转分化的影响

Effects of Cicada Aqueous Extract on the Transdifferentiation of Renal Tubular Epithelial Cells Induced with High Glucose

关键词:  金蝉花水提物  糖尿病肾病  肾间质纤维化

Key words:  cicada aqueous extract  diabetic nephropathy  renal interstitial fibrosis

摘要:

目的 研究金蝉花水提物对高糖诱导下肾小管上皮细胞（HK-2）发生上皮-间充质细胞转分化的影响。方法 采用高糖诱导HK-2细胞建立糖尿病肾病模型，将细胞分为正常组，高糖组，贝那普利组，金蝉花水提物低、高剂量组（0.1，0.5 mg·L^-1），培养24 h后，ELISA测定转化生长因子β1（transforming growth factor-β1，TGF-β1）、纤维连接蛋白（fibronectin，FN），Western blot测定α-平滑肌肌动蛋白（α-smooth muscle actin，α-SMA）的表达情况。结果 与正常组比，高糖组TGF-β1、α-SMA和FN蛋白表达显著增高（P<0.01）；与高糖组比，贝那普利组和金蝉花水提物高剂量组TGF-β1、α-SMA和FN蛋白表达显著降低（P<0.01）。结论 金蝉花水提物可能通过降低TGF-β1、α-SMA和FN蛋白的表达，从而缓解甚至逆转肾小球的硬化与肾间质细胞纤维化，发挥治疗糖尿病肾病的作用。

Abstract:

OBJECTIVE To study the effect of cicada aqueous extract on epithelial-mesenchymal transition of renal tubular epithelial cells(HK-2) induced by high glucose. METHODS HK-2 cells induced by high glucose were used to establish diabetic nephropathy model. The cells were divided into normal group, high glucose group, benazepril group, and cicada aqueous extract low and high dose group(0.1, 0.5 mg·L^-1). After 24 hours of culture, the expression of TGF-β1 and FN were determined by ELISA, α-SMA was determined by Western blot. RESULTS Compared with the normal group, the expression of α-SMA, TGF-β1 and FN protein in the high glucose group was significantly increased(P<0.01). Compared with high glucose group, the indexs in benazepril group and cicada aqueous extract high dose group were significantly decreased(P<0.01). CONCLUSION Cicada aqueous extract may reduce the expression of TGF-β1, α-SMA and FN proteins, so as to relieve or even reverse glomerular sclerosis and renal interstitial cell fibrosis and achieve the effect of treating diabetic nephropathy.