樟芝多糖辅助bFGF对于大鼠机械性脊髓损伤后神经功能修复的作用研究

Study on the Effect of bFGF Combined with Antrodia Camphorate Polysaccharide on the Repair of Neural Function After Mechanical Spinal Cord Injury

关键词:  樟芝多糖  脊髓损伤  神经修复  碱性成纤维细胞因子

Key words:  Antrodia camphorate polysaccharide  spinal cord injury  nerve repair  bFGF

摘要:

目的 研究樟芝多糖联合碱性成纤维细胞因子（basic fibroblast growth factor，bFGF）对于机械性脊髓损伤的大鼠神经功能修复的作用。方法 采用改良的Allens法构建大鼠脊髓机械性损伤模型，将大鼠分为对照组、模型组、bFGF组、樟芝多糖组、bFGF+樟芝多糖组，药物干预时间为30 d。在药物干预后第1，7，14，21，30天对大鼠进行脊髓损伤（Basso-Beattie-Bresnahan，BBB）评分、诱发电位（somatosensory evoked potential，SEP）试验、运动诱发电位（motor evokedpotential，MEP）试验，30 d后处死大鼠，提取脊髓组织后进行HE染色和Nissl染色，Elisa检测组织中炎症因子肿瘤坏死因子（TNF-α）、白介素1β（IL-1β）、趋化因子10（CXCL-10）、集落刺激因子（GM-CSF）和白介素10（IL-10）的表达。结果 bFGF、樟芝多糖单一使用对小鼠神经功能BBB评分和SEP、MEP试验结果均优于模型组（P<0.05），而bFGF+樟芝多糖组的BBB评分和SEP、MEP试验优于单一用药组（P<0.05）。bFGF组、樟芝多糖组、bFGF+樟芝多糖组的脊髓组织中炎症因子TNF-α、IL-1β、CXCL-10的表达显著低于模型组（P<0.05），而GM-CSF和IL-10的表达高于模型组（P<0.05），且bFGF+樟芝多糖组显著优于bFGF组和樟芝多糖组（P<0.05）。结论 樟芝多糖辅助bFGF对于机械性脊髓损伤大鼠有着良好的神经功能修复作用，且优于使用单一的bFGF，其作用可能与抗炎症因子的表达，促进神经细胞的存活有关。

Abstract:

OBJECTIVE To study the effect of bFGF combined with Antrodia camphorate polysaccharide on the repair of neural function after mechanical spinal cord injury. METHODS The mechanical spinal cord injury rats model was established by modified Allens method. The rats were divided into control group, model group, bFGF group, polysaccharide group and bFGF+polysaccharide group. The drug intervention time was 30 days. The Basso-Beattie-Bresnahan (BBB) score, somatosensory evoked potential (SEP) test and motor evoked potential (MEP) experiment were performed on 1, 7, 14, 21, 30 d after drug intervention. After 30 d, the rats were executed for HE staining and Nissl staining with spinal cord tissue. The expression of TNF-α, IL-1β, CXCL-10, GM-CSF and IL-10 in the tissue were detected by Elisa. RESULTS BFGF and polysaccharides alone were superior to model group in BBB score and SEP and MEP test results(P<0.05), the combined group (bFGF+polysaccharide) in BBB score and SEP and MEP test were better than those of the single medication group(P<0.05). The expression of inflammatory factors TNF-α, IL-1β, and chemokine CXCL-10 in spinal cord tissue of bFGF group, polysaccharide group and bFGF+polysaccharide group were significantly lower than those of model group(P<0.05). The expression of GM-CSF and IL-10 was higher than that of model group(P<0.05). The bFGF+polysaccharide group was significantly better than the bFGF group and the polysaccharide group(P<0.05). CONCLUSION bFGF combined with Antrodia camphorate polysaccharide can improve the neural function in mechanical spinal cord injury, the mechanism may be related to the expression of anti-inflammatory factors and the survival of nerve cells.