| 引用本文: | 李波,雷珊珊,李贺,陈烨辉,郑祥,陈奕公,钱柳青,吕圭源,陈素红.基于网络药理学的济脉通片降压机制研究[J].中国现代应用药学,2019,36(1):49-57. |
| LI Bo,LEI Shanshan,LI He,CHEN Yehui,ZHENG Xiang,CHEN Yigong,QIAN Liuqing,LYU Guiyuan,CHEN Suhong.Systematic Understanding the Mechanisms of Ji Mai Tong Tablet Treatment on Hypertension[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(1):49-57. |
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| 基于网络药理学的济脉通片降压机制研究 |
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李波1, 雷珊珊1, 李贺1, 陈烨辉1, 郑祥2, 陈奕公1, 钱柳青1, 吕圭源1,3, 陈素红1,2
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1.浙江工业大学, 杭州 310014;2.温州医科大学, 浙江 温州 325035;3.浙江中医药大学, 杭州 310053
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| 摘要: |
| 目的 采用网络药理学阐明济脉通片多成分-多靶点-多途径的作用理念，为进一步研究济脉通片降压药效物质基础和机制提供一定理论参考。方法 通过TCMSP数据库，结合口服利用度（≥ 30%）和类药性分析（≥ 0.18）参数，筛选济脉通片的活性成分；通过Drugbank和TCMSP数据库进行靶点预测分析；通过GENCARD数据库筛选出高血压疾病相关基因；结合DAVID和KEGG数据库进行GO分析和通路分析；使用Cystoscope软件构建"化合物-靶点-作用通路"网络图。结果 经筛选后得到济脉通片的33个化合物，148个潜在靶基因并映射到了223条信号通路，其中31条信号通路与高血压的发生发展密切相关，其中AGE-RAGE signaling pathway in diabetic complications、PI3K-Akt signaling pathway、TNFsignaling pathway、Adrenergic signaling in cardiomyocytes和Focal adhesion为重要的通路枢纽。结论 济脉通片主要通过多成分、多靶点、多通路调节血管内皮功能、炎症反应、钙钠离子转运、糖脂代谢等参与血管舒张、改善炎症、调节机体代谢、调节离子转运等而产生降压作用。 |
| 关键词: 济脉通片 网络药理学 高血压 血管内皮功能 糖脂代谢 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.01.011 |
| 分类号:R284.1 |
| 基金项目:"重大新药创制"国家科技重大专项（2011ZX09101-002-07）；浙江省重点研发计划（2017C03052）；国家自然科学基金面上项目（81673638，81873036）；国家自然科学基金青年项目（81803760）；中国博士后科学基金（2018M632508） |
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| Systematic Understanding the Mechanisms of Ji Mai Tong Tablet Treatment on Hypertension |
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LI Bo1, LEI Shanshan1, LI He1, CHEN Yehui1, ZHENG Xiang2, CHEN Yigong1, QIAN Liuqing1, LYU Guiyuan1,3, CHEN Suhong1,2
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1.Zhejiang University of Technology, Hangzhou 310014, China;2.Wenzhou Medical University, Wenzhou 325035, China;3.Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, China
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| Abstract: |
| OBJECTIVE To elucidate multicomponent-multi-target-multi-channel analysis by network pharmacology, so as to provide a theoretical reference for further study of the mechanism of antihypertensive action of Ji Mai Tong tablet. METHODS By the TCMSP database, combined with oral bioavailability (≥ 30%) analysis and resistance (≥ 0.18), screening of active ingredients in Ji Mai Tong tablet. Target prediction analysis was carried out by Drugbank and TCMSP database, and the related genes of hypertension disease were screened through GENCARD database. DAVID and KEGG database were combined to carry out GO analysis and path analysis, and Cystoscope software was used to construct the network of compound-target-path. RESULTS The 33 compounds were obtained and 148 number of related potential target genes, which could map to 223 signalling pathways. Among these pathways, 31 pathways were closely related to the development of hypertension. AGE-RAGE signaling pathway in diabetic complications, PI3K-Akt signaling pathway, TNF signaling pathway, Adrenergic signaling in cardiomyocytes and Focal adhesion were the hinge of the whole hypotensive mechanism of Ji Mai Tong tablet. CONCLUSION It is indicated that Ji Mai Tong tablets could regulate vascular endothelial function, inflammatory reaction, calcium and sodium transport, glucose and lipid metabolism and other involved in vasodilation, and then improve inflammation, regulate the body's metabolism, medulating the transport of ions and so on, resulting in antihypertensive effect. |
| Key words: Ji Mai Tong network pharmacology hypertension vascular endothelial function glycolipid metabolism |
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