毛细管微乳电动色谱法的研究进展

Research Progress of Microemulsion Electrokinetic Chromatography

关键词:  微乳电动色谱  药物分析  微乳  手性分离

Key words:  microemulsion electrokinetic chromatography  pharmaceutical analysis  microemulsions  chiral separation

摘要:

目的 介绍近年来毛细管微乳电动色谱法的研究和应用进展，为该技术在药物分析中的应用提供参考。方法 通过检索阅读毛细管微乳电动色谱的文献报道，对微乳电动色谱在正辛醇-水分配系数（logP）测定、天然产物和手性化合物分离等方面的应用进行总结。结果 毛细管微乳电动色谱对于难溶性物质和复杂混合物的分离具显著优势，同时是目前报道的测定logP值最为简便快速准确的色谱方法。微乳液的微观结构分析尚需进一步深入研究。结论 毛细管微乳电动色谱兼具色谱和电泳分离的特点，应用范围较广，可以同时分离水溶性、脂溶性、酸性、碱性及中性物质。但毛细管微乳电动色谱分析的稳定性和重复性还有待提高。

Abstract:

OBJECTIVE To introduce the research progress and application developments of capillary microemulsion electrokinetic chromatography(MEEKC) in recent years, and provide an useful reference for the application of this technology in drug analysis. METHODS Retrieving and reading research reports of MEEKC to summarize the applications of microemulsion electrokinetic chromatography in the determination of N-octanol-water partition coefficient (logP), separation of natural products and chiral compounds. RESULTS MEEKC had significant advantages for the separation of poorly soluble substances and complex mixtures. In the meanwhile, it was one of the most convenient and accurate chromatographic method for determining logP values. However, microstructure analysis of microemulsion still need further research. CONCLUSION MEEKC combines the features of both chromatographic and electrophoretic separations. It has a wide range of application scope and can separate water-soluble, fat-soluble, acidic, basic and neutral substances simultaneously. Furthermore, the stability and reproducibility of capillary microemulsion electrochromatographic analysis still need to be improved.