HPLC测定脑心清流膏及其片剂中槲皮苷的含量

Content Determination of Quercitrin in Naoxinqing Crude Drug and Tablets by HPLC

关键词:  脑心清流膏  脑心清片  槲皮苷  高效液相色谱法  含量测定

Key words:  Naoxinqing crude drug  Naoxinqing tablets  quercitrin  HPLC  content determination

摘要:

目的 建立HPLC测定脑心清流膏及脑心清片中槲皮苷含量的方法。方法 色谱柱为Agilent Eclipse Plus C₁₈柱（250 mm×4.6 mm，5 μm）；以乙腈（A）-0.1%磷酸水溶液（B）为流动相进行梯度洗脱，0~40 min，7%→ 25% A，40~60 min，25%→ 50% A；流速为1.0 mL·min^-1；检测波长为360 nm；柱温为30℃。结果 槲皮苷浓度在19.4~775.7 μg·mL^-1内与峰面积线性关系良好（r=0.999 9），脑心清流膏和脑心清片平均加样回收率（n=6）分别为100.07%和100.16%，RSD分别为1.88%和1.45%。结论 该方法操作简便、结果准确、重现性好，可作为脑心清流膏及其片剂中槲皮苷的含量测定方法。

Abstract:

OBJECTIVE To establish a method of determine the content of quercitrin in Naoxinqing crude drug and tablets by HPLC. METHODS The samples were separated on an Agilent Eclipse Plus C₁₈ column (250 mm×4.6 mm, 5 μm), by a gradient elution using the mobile phase of acetonitrile (A)-0.1% phosphoric acid (B) (0~40 min, 7%→ 25%A; 40~60 min, 25%→ 50%A) at a flow rate of 1.0 mL·min^-1. The detection wavelength was set on 360 nm and the column temperature was set at 30℃. RESULTS A good linearity was observed for quercitrin in the range of 19.4-775.7 μg·mL^-1(r=0.999 9). The average recoveries(n=6) of Naoxinqing crude drug and tablets were 100.07% and 100.16% with the RSD of 1.88% and 1.45%, respectively. CONCLUSION The method is simple, accurate, reproducible, and applicable for the content determination of quercitrin in Naoxinqing crude drug and tablets.