增液汤的网络靶标预测及作用机制研究

Study on Network Target Prediction and Action Mechanism of Zengye Decoction

关键词:  增液汤  网络药理学  聚类分析  蛋白互作

Key words:  Zengye decoction  network pharmacology  cluster analysis  protein interaction

摘要:

目的 采用网络药理学方法对增液汤中活性成分的作用机制进行分析，探讨其成分、靶点及通路间的相关关系。方法 运用中药系统药理学成分分析平台（BAT-MAN）数据库获取增液汤的作用靶标基因，采用Cytoscape 3.5.1软件构建活性成分-靶点网络，STRING平台构建靶蛋白互作网络，利用BiNGO和MCODE插件对相互作用的靶基因进行GO生物过程富集和聚类分析，借助生物学信息注释数据库对靶点进行KEGG信号通路分析。结果 增液汤中23个药效成分作用于268个潜在靶点，聚类分析862个GO生物过程得到物质合成代谢、氧化应激、细胞离子平衡、炎症反应、凝血调节等23个子簇。KEGG信号通路富集主要涉及代谢、神经递质、氨基酸合成、信号转导、心血管和其他等6大类22条信号通路。结论 本研究揭示了增液汤活性成分、靶点及多维药理作用机制，为其临床治疗价值拓展提供新的线索。

Abstract:

OBJECTIVE Using the method of network pharmacology to analyze the mechanism of active ingredients in Zengye decoction, and to explore the correlation among components, target points and pathways. METHODS The traditional Chinese medicine pharmacology component analysis system platform(BAT-MAN) database to obtain the target gene of Zengye decoction. Cytoscape 3.5.1 software was used to build the active component-target network, and the STRING platform was used to build the target protein interaction network. GO bioprocess enrichment and clustering to analysize of interacting target genes by BiNGO and MCODE plug-ins. With the help of the biological information database annotation(DAVID) analysis of KEGG signal pathway on target. RESULTS The 23 pharmacodynamics components in Zengye decoction acted on 268 potential targets, and the cluster analysis of 862 GO biological processes yielded 23 sub-clusters, such as substance synthesis metabolism, oxidative stress, cell ion balance, inflammatory reaction, and coagulation regulation. The enrichment of KEGG signal pathways mainly involved 22 main signal pathways, such as metabolism, neurotransmitters, amino acid synthesis, signal transduction, cardiovascular and others. CONCLUSION This study reveals the active ingredients, target and multi-dimensional pharmacological mechanism of Zengye decoction, and provides new clues for the development of its clinical therapeutic value.