| 引用本文: | 张文婷,史煜华,袁颖,黄琴伟,周建良,郭增喜,马临科.天然没药与胶质没药HPLC特征图谱研究[J].中国现代应用药学,2019,36(3):327-330. |
| ZHANG Wenting,SHI Yuhua,YUAN Ying,HUANG Qinwei,ZHOU Jianliang,GUO Zengxi,MA Linke.Study on HPLC Characteristic Chromatograms of Myrrh and Gum opoponax[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(3):327-330. |
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| 天然没药与胶质没药HPLC特征图谱研究 |
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张文婷1, 史煜华2, 袁颖3, 黄琴伟1, 周建良1, 郭增喜1, 马临科1
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1.浙江省食品药品检验研究院, 杭州 310052;2.浙江中医药大学, 杭州 310052;3.衢州南孔中药有限公司, 浙江 衢州 324000
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| 摘要: |
| 目的 建立天然没药与胶质没药高效液相色谱特征图谱鉴别方法。方法 采用Eclipse Plus C18（250 mm×4.6 mm，5 μm）色谱柱，以乙腈-0.1%磷酸为流动相，梯度洗脱，流速1.0 mL·min-1，检测波长240 nm，建立天然没药与胶质没药的特征图谱，统计分析比较2种没药的差别。结果 建立了天然没药特征图谱，标定7个特征峰；建立了胶质没药特征图谱，标定2个特征峰。2种没药的成分存在明显差异。结论 所建立的2种没药的高效液相色谱特征图谱重复性好，专属性强，可用于2种没药的鉴别。 |
| 关键词: 天然没药 胶质没药 特征图谱 高效液相色谱法 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.03.014 |
| 分类号:R284.1 |
| 基金项目: |
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| Study on HPLC Characteristic Chromatograms of Myrrh and Gum opoponax |
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ZHANG Wenting1, SHI Yuhua2, YUAN Ying3, HUANG Qinwei1, ZHOU Jianliang1, GUO Zengxi1, MA Linke1
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1.Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China;2.Zhejiang Chinese Medical University, Hangzhou 310052, China;3.Quzhou Nankong Chinese Traditional Drugs Co., Quzhou 324000, China
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| Abstract: |
| OBJECTIVE To establish HPLC characteristic chromatograms of Myrrh and Gum opoponax. METHODS The characteristic chromatograms of Myrrh and Gum opoponax were established by HPLC method. The separation was performed using Eclipse Plus C18(250 mm×4.6 mm, 5μm) column by gradient elution with acetonitrile and 0.1% phosphoric acid aqueous solution as the mobile phase at a flow rate of 1.0 mL·min-1. The UV detection wavelength was set at 240 nm. Statistical analysis was applied to evaluate the difference between Myrrh and Gum opoponax. RESULTS The HPLC characteristic chromatograms of Myrrh and Gum opoponax were established, in which seven and two characteristic peaks were assigned, respectively. There were significant differences between Myrrh and Gum opoponax. CONCLUSION The established HPLC characteristic chromatograms of Myrrh and Gum opoponax showed good reproducibility and specificity, and can be used for the identification of Myrrh and Gum opoponax. |
| Key words: Myrrh Gum opoponax characterastic chromatogram HPLC |
