甘草超滤液中甘草苷的络合萃取研究

Study on Complex Extraction of Liquiritin from Glycyrrhizae Radix Et Rhizoma Ultrafiltrate

关键词:  甘草苷  络合萃取  络合剂  稀释剂  甘草超滤液

Key words:  liquiritin  complexation extraction  complexant  thinner  Glycyrrhizae Radix Et Rhizoma ultrafiltrate

摘要:

目的 通过研究络合萃取剂组成变化对甘草超滤液中甘草苷萃取效果的影响，优选出适合于甘草苷萃取分离的络合萃取剂。方法 以甘草苷和甘草酸单次萃取率为指标，通过单因素实验，在考察多种二元混合络合萃取剂对甘草超滤液中指标成分萃取情况的基础上，采用U₅（5³）均匀设计优化含有2种络合剂的三元络合萃取剂，以期进一步提高甘草苷络合萃取效率，降低成本。结果 三元络合萃取剂中磷酸三丁酯（tributyl phosphate，TBP）对三烷基氧化膦（trialkyphosphine oxide，TRPO）络合萃取甘草苷没有协同萃取作用。优选15% TRPO+85%磺化煤油所组成的络合萃取剂甘草苷的单次萃取率为99.6%，甘草酸的单次萃取率为8.3%。结论 优选的络合萃取剂对甘草超滤液中甘草苷的萃取能力强、选择性高，可用于甘草超滤液中甘草苷的络合萃取。

Abstract:

OBJECTIVE To select out a complexing extracting agent suitable for extracting and separating the liquiritin, and study the effect of composition changes of complex extractant on the extraction of liquiritin from Glycyrrhizae Radix Et Rhizoma ultrafiltrate. METHODS The single extraction rate of liquiritin and glycyrrhizic acid were taken by the complex extractant as an index, through single factor experiments, on the basis of investigating the extraction of index components in licorice ultrafiltrate by a variety of binary complexing extractants. U₅(5³) was adopted to uniformly design and optimize ternary complexing extractants containing two complexing agents, to further improve the extraction efficiency of liquiritin complexing and reduce costs. RESULTS Tributyl phosphate (TBP) in ternary complex extractant had no synergistic extraction effect on trialkyl phosphine oxide (TRPO) complex extraction of liquiritin. Preferably, the single extraction rate of liquiritin by the complex extractant composed of 15% TRPO + 85% sulfonated kerosene was 99.6%, and the single extraction rate of glycyrrhizic acid by the complex extractant was 8.3%. CONCLUSION The optimized complexing extractant has strong extraction capacity and high selectivity for liquiritin in Glycyrrhiza ultrafiltrate, and can be used for complexing extraction of liquiritin in Glycyrrhiza ultrafiltrate.