ICP-MS分析丽水地产灵芝中的重金属元素

Determination of Heavy Metals in Ganoderma from Lishui by ICP-MS

关键词:  灵芝  金属元素  电感耦合等离子体质谱法

Key words:  Ganoderma  metal elements  inductively coupled plasma mass spectrometry(ICP-MS)

摘要:

目的 采用电感耦合等离子体质谱法（ICP-MS）建立灵芝中砷（As）、汞（Hg）、铅（Pb）、镉（Cd）、铜（Cu）5种重金属元素的测定方法。方法 采用微波消解仪进行消解，以¹¹⁵In、⁷²Ge、²⁰⁹Bi为内标，采用ICP-MS法测定丽水产地灵芝的菌盖与菌柄中As、Hg、Pb、Cd、Cu的含量。结果 检测的5种元素线性关系良好，相关系数r ≥ 0.999 1。22批样品中Cd超出限度的有5批，As、Pb超出限度的各有1批，Cu超出限度的有2批。Cu、Cd、Hg在菌盖中的富集较多，As、Pb则在菌柄中富集较明显。结论 该方法灵敏、高效、准确，且分别测定了灵芝不同药用部位的重金属含量，对龙泉灵芝栽培基地选择，人工种植过程中重金属含量控制等具有现实意义，可用于灵芝的安全性评价。

Abstract:

OBJECTIVE To detect 5 heavy metal elements including As, Hg, Pb, Cd and Cu in Ganoderma by inductively coupled plasma mass spectrometry(ICP-MS). METHODS The content of As, Hg, Pb, Cd and Cu in Ganoderma from Lishui were determined by ICP-MS using ¹¹⁵In, ⁷²Ge and ²⁰⁹Bi as internal standard by microwave digestion. RESULTS The linearity of the 5 elements were good, and the correlation were ≥ 0.999 1. There were 5 batches of Cd exceeding the limit in the 22 batches of samples, one batch of As and Pb exceeding the limit, and 2 batches of Cu exceeding the limit. Cu, Cd and Hg accumulated more in the cap, while As and Pb accumulated more in the stalk. CONCLUSION The method is sensitive, efficient and accurate, and the content of heavy metals in different medicinal parts of Ganoderma are determined respectively. It has practical significance to select Ganoderma cultivation base in Longquan, and control the heavy metal content during antificial planting, etc. It can be used for the safety evaluation of Ganoderma.